Can established cultured papilloma cells harbor bovine papillomavirus?

Autor(es):Mostrar menos -

Campos, S. R. C. ^[1] ; Trindade, C. ^[1] ; Ferraz, O. P. ^[1] ; Giovanni, D. N. S. ^[1] ; Lima, A. A. ^{[2, 1]} ; Caetano, H. V. A. ^{[3, 1]} ; Carvalho, R. F. ^{[3, 1]} ; Birgel, Jr., E. H. ^[4] ; Dagli, M. L. Z. ^[3] ; Mori, E. ^[5] ; Brandao, P. E. ^[5] ; Richtzenhain, L. J. ^[5] ; Becak, W. ^{[6, 1]} ; Stocco, R. C. ^[1]

Afiliação do(s) autor(es)

^[1] Inst Butantan, Genet Lab, Sao Paulo, SP - Brazil

^[2] Univ Fed Ouro Preto, Escola Farm, Ouro Preto, MG - Brazil

^[3] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin Med, Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, Sao Paulo - Brazil

^[6] Univ Fed Pernambuco, Dept Genet, Recife, PE - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 7, n. 4, p. 1119-1126, 2008.
• Citações Web of Science: 5

Resumo

Papillomaviruses have been reported to be very difficult to grow in cell culture. Also, there are no descriptions of cell cultures from lesions of bovine cutaneous papillomatosis, with identification of different bovine papilloma virus (BPV) DNA sequences. In the present report, we describe primary cell cultures from samples of cutaneous lesions (warts). We investigated the simultaneous presence of different BPV DNA sequences, comparing the original lesion to different passages of the cell cultures and to peripheral blood. BPV 1, 2 and 4 DNA sequences were found in lesion samples, and respective cell cultures and peripheral blood, supporting our previous hypothesis of the possible activity of these sequences in different samples and now also showing how they can be maintained in different passages of cell cultures. (AU)

• Processo FAPESP: 06/02439-6 - Interação papilomavírus bovino-genoma da célula hospedeira: avaliação da expressão do genoma viral em sangue e/ou linfócitos periféricos
• Beneficiário: Rita de Cassia Stocco
• Modalidade de apoio: Auxílio à Pesquisa - Regular