| Texto completo | |
| Autor(es): |
Paixaeo, Thiago R. L. C.
[1]
;
Barbosa, Livea F.
[1]
;
Carri, Maria T.
[2, 3]
;
Medeiros, Marisa H. G.
[1]
;
Bertotti, Mauro
[1]
Número total de Autores: 5
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Quim, BR-05508900 Sao Paulo - Brazil
[2] Univ Roma Tor Vergata, Dept Biol, I-00133 Rome - Italy
[3] Fdn Santa Lucia IRRCS, Rome - Italy
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ANALYST; v. 133, n. 11, p. 1605-1610, 2008. |
| Citações Web of Science: | 11 |
| Resumo | |
The uptake of ascorbate by neuroblastoma cells using a ruthenium oxide hexacyanoferrate (RuOHCF)-modified carbon fiber disc (CFD) microelectrode (r = 14.5 mu m) was investigated. By use of the proposed electrochemical sensor the amperometric determination of ascorbate was performed at 0.0 V in minimum essential medium (MEM, pH = 7.2) with a limit of detection of 25 mu mol L(-1). Under the optimum experimental conditions, no interference from MEM constituents and reduced glutathione (used to prevent the oxidation of ascorbate during the experiments) was noticed. The stability of the RuOHCF-modified electrode response was studied by measuring the sensitivity over an extended period of time (120 h), a decrease of around 10% being noticed at the end of the experiment. The rate of ascorbate uptake by control human neuroblastoma SH-SY5Y cells, and cells transfected with wild-type Cu,Zn-superoxide dismutase (SOD WT) or with a mutant typical of familial amyotrophic lateral sclerosis (SOD G93A), was in agreement with the level of oxidative stress in these cells. The usefulness of the RuOHCF-modified microelectrode for in vivo monitoring of ascorbate inside neuroblastoma cells was also demonstrated. (AU) | |
| Processo FAPESP: | 07/04955-4 - Construcao de linguas eletronicas voltametricas visando ao desenvolvimento de dispositivos inteligentes. |
| Beneficiário: | Thiago Regis Longo Cesar da Paixão |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 03/13757-0 - Fabricacao e utilizacao de microeletrodos para determinacoes amperometricas em micro-ambientes. |
| Beneficiário: | Thiago Regis Longo Cesar da Paixão |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |