Characterization of the insulinotropic action of a phospholipase A(2) isolated from Crotalus durissus collilineatus rattlesnake venom on rat pancreatic islets

Autor(es)

Nogueira, Tatiane C. A. ^[1] ; Ferreira, Fabiano ; Toyama, Marcos H. ; Stoppiglia, Luiz Fabrizio ; Marangoni, Sergio ; Boschero, Antonio C. ; Carneiro, Everardo M.

Afiliação do(s) autor(es)

^[1] Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 45, n. 2, p. 243-248, Feb. 2005.
• Área do conhecimento: Ciências Biológicas - Fisiologia
• Assunto(s): Ilhotas pancreáticas Fosfolipases Crotoxina Venenos de origem animal Crotalus

Resumo

The ability of PLA2 and crotapotin, isolated from Crotalus durissus collilineatus rattlesnake venom, to stimulate insulin secretion from isolated rat islets was examined. PLA2 and crotapotin stimulated insulin secretion at 2.8 mmol/L glucose, whereas at a high glucose concentrations (16.7 mmol/L) only PLA2 stimulated secretion. Nifedipine (10 ìmol/L) did not alter the ability of PLA2 to increase insulin secretion stimulated by a depolarizing concentration of K+ (30 mmol/L). PLA2 did not affect 14CO2 production but significantly increased the efflux of arachidonic acid from isolated islets. These results indicate that PLA2-stimulated secretion is not dependent on an additional influx of Ca2+ through L-type Ca2+-channels but rather is associated with arachidonic acid formation in pancreatic islets. (AU)

• Processo FAPESP: 00/04822-5 - Modulacao da funcao cardiaca e do mecanismo de secrecao de insulina pela neurotoxina crotoxina-like de crotalus durissus collilineatus em ratos jovens.
• Beneficiário: Tatiane Cristina de Araujo Nogueira
• Modalidade de apoio: Bolsas no Brasil - Mestrado