Hormonal modulation of photomorphogenesis-controlled anthocyanin accumulation in tomato (Solanum lycopersicum L. cv Micro-Tom) hypocotyls: Physiological and genetic studies

Autor(es)

Carvalho, Rogerio Falleiros ^[1] ; Quecini, Vera ^[2] ; Pereira Peres, Lazaro Eustaquio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Ciencias Biol, LCB, ESALQ, BR-13418900 Piracicaba, SP - Brazil

^[2] EMBRAPA, CNPUV, BR-95700000 Bento Goncalves, RS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Plant Science; v. 178, n. 3, p. 258-264, MAR 2010.
• Citações Web of Science: 17

Resumo

Hormones are likely to be important factors modulating the light-dependent anthocyanin accumulation. Here we analyzed anthocyanin contents in hypocotyls of near isogenic Micro-Tom (MT) tomato lines carrying hormone and phytochrome mutations, as single and double-mutant combinations. In order to recapitulate mutant phenotype, exogenous hormone applications were also performed Anthocyanin accumulation was promoted by exogenous abscisic acid (ABA) and inhibited by gibberellin (GA), in accordance to the reduced anthocyanin contents measured in ABA-deficient (notabills) and GA-constitutive response (procera) mutants. Exogenous cytokinin also enhanced anthocyanin levels in MT hypocotyls. Although auxin-insensitive chageotropica mutant exhibited higher anthocyanin contents, pharmacological approaches employing exogenous auxin and a transport inhibitor did not support a direct role of the hormone in anthocyanin accumulation Analysis of mutants exhibiting increased ethylene production (epwastic) or reduced sensitivity (Never ripe), together with pharmacological data obtained from plants treated with the hormone, indicated a limited role for ethylene in anthocyanin contents. Phytochrome-deficiency (aurea) and hormone double-mutant combinations exhibited phenotypes suggesting additive or synergistic interactions, but not fully espistatic ones, in the control of anthocyanin levels in tomato hypocotyls. Our results indicate that phytochrome-mediated anthocyanin accumulation in tomato hypocotyls is modulated by distinct hormone classes via both shared and independent pathways. (C) 2010 Elsevier Ireland Ltd. All rights reserved (AU)

• Processo FAPESP: 02/00329-8 - Estudo das bases genéticas e bioquímicas da competência para regeneração in vitro utilizando micro tomateiro (Lycopersicon esculentum cv Micro-Tom) como modelo experimental
• Beneficiário: Lázaro Eustaquio Pereira Peres
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 03/12416-5 - Estudo da interação entre fitocromo e hormônios vegetais no controle do desenvolvimento
• Beneficiário: Rogério Falleiros Carvalho
• Modalidade de apoio: Bolsas no Brasil - Doutorado