Differential display and suppression subtractive hybridization analysis of the pulp of ripening banana

Autor(es)

de Godoy, Adriana ^[1] ; Cordenunsi, Beatriz Rosana ^[1] ; Lajolo, Franco Maria ^[1] ; Oliveira do Nascimento, Joao Roberto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, FCF, Dept Alimentos & Nutr Expt, Lab Quim Bioquim & Biol Mol Alimentos, BR-05508900 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Scientia Horticulturae; v. 124, n. 1, p. 51-56, FEB 26 2010.
• Citações Web of Science: 3

Resumo

The fruit of banana undergoes several important physico-chemical changes during ripening. Analysis of gene expression would permit identification of important genes and regulatory elements involved in this process. Therefore, transcript profiling of preclimacteric and climacteric fruit was performed using differential display and Suppression subtractive hybridization. Our analyses resulted in the isolation of 12 differentially expressed cDNAs, which were confirmed by dot-blots and northern blots. Among the sequences identified were sequences homologous to plant aquaporins, adenine nucleotide translocator, immunophilin, legumin-like proteins, deoxyguanosine kinase and omega-3 fatty acid desaturase. Some of these cDNAs correspond to newly isolated genes involved in changes related to the respiratory climacteric, or stress-defense responses. Functional characterization of ripening-associated genes could provide information useful in controlling biochemical pathways that would have an impact on banana quality and shelf life. (C) 2009 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 02/12452-9 - Metabolismo de carboidratos durante o amadurecimento de frutos: aplicações da genômica funcional
• Beneficiário: Franco Maria Lajolo
• Modalidade de apoio: Auxílio à Pesquisa - Temático