Determination of isoniazid in human urine using screen-printed carbon electrode modified with poly-L-histidine

Autor(es)

Bergamini, Marcio F. ^[1] ; Santos, Daniela P. ^[2] ; Zanoni, Maria Valnice B. ^[2]

Afiliação do(s) autor(es)

^[1] UFPR, Ctr Politecn, Dept Quim, Setor Ciencias Exatas, BR-81531990 Curitiba, Parana - Brazil

^[2] UNESP, Inst Chem, BR-14801970 Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOELECTROCHEMISTRY; v. 77, n. 2, p. 133-138, FEB 2010.
• Citações Web of Science: 61

Resumo

A sensitive voltammetric method for trace measurements of isoniazid (INZ) in synthetic human urine sample is described. The method is based on its electroreduction at -0.98 V vs. Ag/AgCl on screen-printed carbon electrode (SPCE) modified with poly-L-histidine (PH). A film of good adherence on SPCE and electrocatalytic properties was obtained coating the electrode by histidine monomer electropolymerization process (SPCE/EPH). The electrochemical behavior of the modified SPCE was investigated by cyclic, linear sweep (LSV), differential pulse (DPV). square-wave voltammetry (SWV), and electrochemical impedance spectroscopy (EIS). Limits of detection of 5.0 x 10(-7) mol L(-1), 1.7 x 10(-7) mol L(-1) and 2.5 x 10(-7) mol L(-1) were estimated from LSV, DPV and SWV determinations, respectively. The method was successfully applied to the determination of INZ in human urine samples. (c) 2009 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 03/06598-3 - Desenvolvimento de eletrodos modificados com poli acido glutamico e sua aplicacao na analise de compostos antioxidantes e farmaceuticos.
• Beneficiário: Daniela Pereira dos Santos
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 04/00111-8 - Desenvolvimento e aplicação de sensores eletroquímicos baseados em filmes poliméricos de poli-L-histidina
• Beneficiário: Marcio Fernando Bergamini
• Modalidade de apoio: Bolsas no Brasil - Doutorado