Testosterone represses ubiquitin ligases atrogin-1 and Murf-1 expression in an androgen-sensitive rat skeletal muscle in vivo

Autor(es)

Pires-Oliveira, Marcelo ^[1] ; Maragno, Ana Leticia G. C. ^[2] ; Parreiras-e-Silva, Lucas T. ^[2] ; Chiavegatti, Tiago ^[1] ; Gomes, Marcelo D. ^[2] ; Godinho, Rosely O. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Pharmacol, BR-04044020 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biochem & Immunol, Ribeirao Preto - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Physiology; v. 108, n. 2, p. 266-273, FEB 2010.
• Citações Web of Science: 32

Resumo

Pires-Oliveira M, Maragno AL, Parreiras-E-Silva LT, Chiavegatti T, Gomes MD, Godinho RO. Testosterone represses ubiquitin ligases atrogin-1 and Murf-1 expression in an androgen-sensitive rat skeletal muscle in vivo. J Appl Physiol 108: 266-273, 2010. First published November 19, 2009; doi:10.1152/japplphysiol.00490.2009.-Skeletal muscle atrophy induced by denervation and metabolic diseases has been associated with increased ubiquitin ligase expression. In the present study, we evaluate the influence of androgens on muscle ubiquitin ligases atrogin-1/MAFbx/FBXO32 and Murf-1/Trim63 expression and its correlation with maintenance of muscle mass by using the testosterone-dependent fast-twitch levator ani muscle (LA) from normal or castrated adult male Wistar rats. Gene expression was determined by qRT-PCR and/or immunoblotting. Castration induced progressive loss of LA mass (30% of control, 90 days) and an exponential decrease of LA cytoplasm-to-nucleus ratio (nuclear domain; 22% of control after 60 days). Testosterone deprivation induced a 31-fold increase in LA atrogin-1 mRNA and an 18-fold increase in Murf-1 mRNA detected after 2 and 7 days of castration, respectively. Acute (24 h) testosterone administration fully repressed atrogin-1 and Murf-1 mRNA expression to control levels. Atrogin-1 protein was also increased by castration up to 170% after 30 days. Testosterone administration for 7 days restored atrogin-1 protein to control levels. In addition to the well known stimulus of protein synthesis, our results show that testosterone maintains muscle mass by repressing ubiquitin ligases, indicating that inhibition of ubiquitin-proteasome catabolic system is critical for trophic action of androgens in skeletal muscle. Besides, since neither castration nor androgen treatment had any effect on weight or ubiquitin ligases mRNA levels of extensor digitorum longus muscle, a fast-twitch muscle with low androgen sensitivity, our study shows that perineal muscle LA is a suitable in vivo model to evaluate regulation of muscle proteolysis, closely resembling human muscle responsiveness to androgens. (AU)

• Processo FAPESP: 06/58629-8 - Mecanismos celulares e moleculares da atrofia muscular induzida pela privacao de androgeno.
• Beneficiário: Marcelo Pires de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/59006-1 - AMP cíclico intra e extracelular como sinalizador da sinapse neuromuscular esquelética: mecanismos de regulação
• Beneficiário: Rosely Oliveira Godinho
• Modalidade de apoio: Auxílio à Pesquisa - Regular