Could the Leukocyte X Chromosome Inactivation Pattern Be Extrapolated to Hair Bulbs?

Autor(es)

Kaupert, Laura C. ^[1] ; Billerbeck, Ana Elisa C. ^[1] ; Brito, Vinicius N. ^[1] ; Mendonca, Berenice B. ^[1] ; Bachega, Tania A. S. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Lab Hormones & Mol Genet LIM 42, Div Endocrinol, Clin Hosp, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Hormone Research in Paediatrics; v. 73, n. 4, p. 238-243, 2010.
• Citações Web of Science: 3

Resumo

Background: The androgen receptor gene is located on the X chromosome with a polymorphic tract of CAG repeats that is inversely correlated to the receptor's transactivation activity. A short CAG tract is associated with hyperandrogenic disorders. In women, one of the X chromosomes is inactivated and the X chromosome inactivation (XCI) pattern varies among tissues. Previous studies of hyperandrogenic disorders only evaluated XCI in leukocytes. Objective: To evaluate whether the XCI pattern in leukocytes could be extrapolated to those in hair bulbs. Material: A total of 58 healthy women were used for this study. DNA was extracted from leukocytes (n = 58 women) and pubic (n = 53 women) and scalp hair (n = 21 women). Methods: Hpa II digested and undigested DNA samples underwent fluorescence PCR GeneScan (R) analysis. Results: A significant and positive correlation of XCI was found between leukocytes and hair bulbs. However, individual comparisons showed that 13 and 19% of the women presented a different leukocyte XCI pattern in pubic hair and similar in leukocytes and hair bulbs of normal women indicating that leukocyte DNA is useful for XCI analysis. However, the XCI pattern could vary among tissues from the same subject, indicating that care should be taken when extrapolating individual leukocyte XCI patterns to other tissue. Copyright (C) 2010 S. Karger AG, Basel (AU)

• Processo FAPESP: 06/56028-7 - Análise da correlação entre o número de repetições CAG no exon 1 do receptor de andrógenos em DNA de bulbo piloso com o grau de virilização da genitália externa em pacientes com a forma clássica da deficiência da 21-hidroxilase em DNA de bulbo piloso e de leucócitos periféricos
• Beneficiário: Laura Cesar Kaupert
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica