Immunolocalization of matrix metalloproteinases-2 and-9 during apical periodontitis development

Autor(es)

Corotti, Mauro V. ^[1] ; Zambuzzi, Willian F. ^[2] ; Paiva, Katiucia B. S. ^[3] ; Menezes, Renato ^{[4, 5]} ; Pinto, Lidiane C. ^[6] ; Lara, Vanessa S. ^[6] ; Granjeiro, Jose M. ^{[7, 8]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Biochem Lab, Dept Biol Sci, Bauru, SP - Brazil

^[2] Univ Estadual Campinas, Lab Bioassays & Signal Transduct, Dept Biochem, Inst Biol, Campinas, SP - Brazil

^[3] Univ Sao Paulo, Lab Mol Pathol, Dept Oral Pathol, Sch Dent, Sao Paulo - Brazil

^[4] Univ Pittsburgh, Sch Dent Med, Dept Oral Biol, Pittsburgh, PA - USA

^[5] Univ Pittsburgh, Sch Dent Med, Ctr Craniofacial & Dent Genet, Pittsburgh, PA - USA

^[6] Univ Sao Paulo, Pathol Lab, Dept Stomatol, Bauru, SP - Brazil

^[7] Univ Fed Fluminense, Dept Cell & Mol Biol, Inst Biol, BR-24020150 Niteroi, RJ - Brazil

^[8] Univ Sao Paulo, Cell & Mol Biol Ctr Nucell, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 54, n. 8, p. 764-771, AUG 2009.
• Citações Web of Science: 40

Resumo

Objective: The objective of this study was to determine the expression of matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) during apical periodontitis development. Methods: Using an experimental design of induced periapical lesions in rats and immunohistochemistry assay as investigative tool, the MMP-2 and MMP-9 expression and distribution were evaluated at 3, 7,14, 21, 30,60 and 90 days after coronary access and pulp exposure of the first left mandibular molar to the oral environment. Two blind observers scored the immunoreactivity. A semi-quantitative analysis was performed. Results: Except at day 3, MMP-2 and MMP-9 immunostaining was observed in all experimental periods. The MMP-2 (p = 0.004) and MMP-9 (p = 0.005) immunostaining was higher in the period between 7 and 21 days. They were mainly observed in cells surrounding the apical foramen and adjacent periapical areas. Cells into the hypercementosis areas were strongly stained while both osteoblasts and osteoclasts; presented discrete staining along of this study. No staining was observed on epithelial walls. At 30, 60 and 90 days, the subjacent connective tissue presented intense MMP-2 and MMP-9 immunostaining in mononuclear cells (suggestive of fibroblasts, macrophages, infiltrating neutrophils and lymphocytes). Conclusion: The results observed in this study suggest that MMP-2 and MMP-9 play a critical role in the development of inflammatory periapical lesions, probably involved in the extracellular matrix (ECM) degradation during the initial phase of the lesion development. (C) 2009 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/53003-9 - Investigação da função da proteína tirosina fosfatase de baixa massa molecular (LMW-PTP) na adesão de osteoblastos e formação óssea
• Beneficiário: Willian Fernando Zambuzzi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado