Characterization of two novel polyfunctional mastoparan peptides from the venom of the social wasp Polybia paulista

Autor(es)

de Souza, Bibiana Monson ^[1] ; Rodrigues da Silva, Alessandra Vaso ^[1] ; Ferreira Resende, Virginia Maria ^[1] ; Arcuri, Helen Andrade ^[2] ; dos Santos Cabrera, Marcia Perez ^[2] ; Ruggiero Neto, Joao ^[2] ; Palma, Mario Sergio ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, CEIS, IBRC, Dept Biol, BR-13506900 Rio Claro, SP - Brazil

^[2] UNESP, IBILCE, Dept Phys, Sao Jose Do Rio Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Peptides; v. 30, n. 8, p. 1387-1395, AUG 2009.
• Citações Web of Science: 41

Resumo

Hymenoptera venoms are complex mixtures of biochemically and pharmacologically active components such as biogenic amines, peptides and proteins. Polycationic peptides generally constitute the largest group of Hymenoptera venom toxins, and the mastoparans constitute the most abundant and important class of peptides in the venom of social wasps. These toxins are responsible for histamine release from mast cells, serotonin from platelets, and catecholamines and adenylic acids from adrenal chromafin cells. The present work reports the structural and functional characterization of two novel mastoparan peptides identified from the venom of the neotropical social wasp Polybia paulista. The mastoparans Polybia-MP-II and -III were purified, sequenced and synthesized on solid phase using Fmoc chemistry and the synthetic peptides used for structural and functional characterizations. Polybia-MP-II and -III are tetradecapeptides, amidated at their C-termini, and form amphipathic alpha-helical conformations under membrane-mimetic conditions. Both peptides were polyfunctional, causing pronounced cell lysis of rat mast cells and erythrocytes, in addition to having antimicrobial activity against both Gram-positive and Gram-negative bacteria. (C) 2009 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 04/07942-2 - A bioprospecção da fauna de artrópodes do estado de São Paulo pela procura de compostos-líderes para o desenvolvimento racional de novos fármacos e pesticidas seletivos
• Beneficiário: Mario Sergio Palma
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular