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(Referência obtida automaticamente do Google Scholar, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Characterization of novel sugarcane expressed sequence tag microsatellites and their comparison with genomic SSRs

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Autor(es):
Pinto‚ LR ; Oliveira‚ KM ; Marconi‚ T. ; Garcia‚ AAF ; Ulian‚ EC ; De Souza‚ AP
Número total de Autores: 6
Tipo de documento: Artigo Científico
Fonte: PLANT BREEDING; v. 125, n. 4, p. 378-384, 2006.
Resumo

Microsatellites or simple sequence repeats (SSRs) are one of the most suitable markers for genome analysis as they have great potential to aid breeders to develop new improved sugarcane varieties. The development of SSR derived from expressed sequence tags (EST) opens new opportunities for genetic investigations at a functional level. In the present work, the polymorphism obtained with a subset of 51 EST-SSRs derived from SUCEST was compared with those generated by 50 genomic SSRs (gSSR) in terms of number of alleles, polymorphism information content, discrimination power and their ability to establish genetic relationships among 18 sugarcane clones including three Saccharum species (S. officinarum, S. barberi, S. sinense). The majority of EST SSRs loci had four to six alleles in contrast to the seven to nine observed for the gSSRs loci. Approximately, 35% of the gSSRs had PIC values around 0.90 in contrast to 15% of the EST-SSRs. However, the mean discrimination power of the two types of SSR did not differ significantly as much as the average genetic similarity (GS) based on Dice coefficient. The correlation between GS of the two types of SSRs was high (r = 0.71/P = 0.99) and significant. Although differences were observed between dendrograms obtained with each SSR type, both were in good agreement with pedigree information. The S. officinarum clone IJ76-314 was grouped apart from the other clones evaluated. The results here demonstrate that EST-SSRs can be successfully used for genetic relationship analysis, extending the knowledge of genetic diversity of sugarcane to a functional level. (AU)

Processo FAPESP: 02/00197-4 - Desenvolvimento de marcadores moleculares do tipo microssatélite a partir de ESTs em cana-de-açúcar de mapeamento do teor de sacarose
Beneficiário:Karine Miranda Oliveira
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 02/01167-1 - Desenvolvimento de marcadores moleculares a partir de ESTs de cana-de-acúcar para seleção de características economicamente importantes
Beneficiário:Anete Pereira de Souza
Modalidade de apoio: Auxílio à Pesquisa - Parceria para Inovação Tecnológica - PITE