Detection of TGIF1 homeobox gene in oral squamous cell carcinoma according to histologic grading

Autor(es)

Matizonkas-Antonio, Luciana F. ; Liborio, Tatiana Nayara ^[1] ; Aquino Xavier, Flavia Calo ; Silva-Valenzuela, Maria das Gracas ^[2] ; Michaluarte-Junior, Pedro ^[3] ; Nunes, Fabio Daumas ^[4]

Afiliação do(s) autor(es)

^[1] Univ Fed Amazonas, Dept Pathol, Sch Med, Amazonas - Brazil

^[2] Univ Sao Paulo, Dept Biochem, Inst Chem, BR-05508000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Head & Neck Surg Serv, Hosp Clin, BR-05508000 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Odontol, Dept Oral Pathol, Sch Dent, BR-05508000 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology; v. 111, n. 2, p. 218-224, 2011.
• Citações Web of Science: 4

Resumo

Objective. TGIF1 homeobox gene involvement in oral cancer has not yet been investigated. This study analyzed the expression of TGIF1 transcripts and protein in oral squamous cell carcinoma (OSCC). Study design. Snap-frozen samples from 16 patients were taken from both OSCC and nontumoral adjacent epithelium (NT) for in situ hybridization (ISH). Forty-six paraffin-embedded samples of OSCC were submitted to immunohistochemistry (IHC). A descriptive analysis of the transcript signal detection was accomplished, and TGIF1 immunoexpression was carried out considering protein levels, localization, and cellular differentiation. Results. ISH reactions showed TGIF1 transcripts with a signal that was frequently intense in NT, and generally weak in OSCC, and that had stronger transcript signal in well-differentiated areas of OSCC when compared with poorly differentiated ones. IHC reactions had poorly differentiated cases associated with TGIF1 protein expression in both the nucleus and cytoplasm (P = .05, Fisher test). Conclusions. TGIF1 gain or loss of function might possibly play a role in oral cancer cell differentiation. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 111: 218-224) (AU)

• Processo FAPESP: 01/13644-6 - Estudo da expressão de genes homeobox em carcinomas epidermoides de boca
• Beneficiário: Fabio Daumas Nunes
• Modalidade de apoio: Auxílio à Pesquisa - Regular