Infectivity of Strongyloides venezuelensis is influenced by variations in temperature and time of culture

Autor(es)

Machado, Eleuza Rodrigues ^[1] ; Ueta, Marlene Tiduko ^[1] ; Lourenco, Elaine Vicente ^[2] ; Anibal, Fernanda de Freitas ^[3] ; Garcia da Silva, Erica Vitalino ^[3] ; Roque-Barreira, Maria Cristina ^[2] ; Costa-Cruz, Julia Maria ^[4] ; Faccioli, Lucia Helena ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Dept Parasitol, Inst Biol, BR-13083970 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular Mol & Bioagentes Patogenicos, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, SP - Brazil

^[4] Univ Fed Uberlandia, Dept Imunol Microbiol & Parasitol, Inst Ciencias Biomed, BR-38400 Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Experimental Parasitology; v. 127, n. 1, p. 72-79, 2011.
• Citações Web of Science: 0

Resumo

The present research investigated the influence of temperature and time of larvae culture on the infectivity of Strongyloides venezuelensis. Mice were infected s.c. with 1500 larvae of S. venezuelensis maintained at 28 degrees C for three days of culture (dc), 28 degrees C for seven dc or 18 degrees C for seven dc. On days 1,3, 5, 7, 14 and 21 post-infection the animals were sacrificed and cell numbers in the blood, peritoneal cavity fluid (PCF), broncoalveolar fluid (BALF), cytokines, immunoglobulins, number of parasites and eggs/g of feces were quantified. Results demonstrated an increase in eosinophils and mononuclear cells in the blood, PCF and HALF of infected mice. Larvae at 28 degrees C/3dc induced earlier eosinophils in the PCF and HALF as opposed to larvae at 28 degrees C/7dc and 18 degrees C/7dc. Larvae at 28 degrees C/7dc induced higher synthesis of IL-4. IL-5 and IL-10 on days Sand 7 post-infection. Larvae at 28 degrees C/3dc in culture induced higher synthesis of IL-12 than larvae of seven dc, but time in culture induced better synthesis of IFN-gamma, after larval migration had ceased and only adult worms were present. Larvae at 28 degrees C/3dc in culture induced higher synthesis of IgG and IgG1 and expelled less female parasites than larvae cultivated for seven days. In conclusion, it was observed that the infectivity of S. venezuelensis is influenced by variations in temperature and time of culture. (C) 2010 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 02/12856-2 - Modulação das respostas imunes inata e adquirida por leucotrienos e prostaglandinas
• Beneficiário: Lúcia Helena Faccioli
• Modalidade de apoio: Auxílio à Pesquisa - Temático