Ursodeoxycholic acid ameliorates experimental ileitis counteracting intestinal barrier dysfunction and oxidative stress

Autor(es)

Bernardes-Silva, Carlos Felipe ; Damião, Adérson O. M. C. ; Sipahi, Aytan M. ; Laurindo, Francisco Rafael Martins ^[4] ; Iriya, Kiyoshi ; Lopasso, Fabio P. ; Buchpiguel, Carlos A. ; Lordello, Maria Laura L. ; Agostinho, Carmem L. O. ; Laudanna, Antonio A.

• Tipo de documento: Artigo Científico
• Fonte: Digestive Diseases and Sciences; v. 49, n. 10, p. 1569-1574, Oct. 2004.
• Área do conhecimento: Ciências da Saúde - Medicina
• Assunto(s): Gastroenteropatias Enteropatias inflamatórias Doença de Crohn Ácido ursodesoxicólico Indometacina Estresse oxidativo

Resumo

The aim of this study was to evaluate the effect of ursodeoxycholic acid (UDCA) on intestinal permeability (IP) and reactive oxygen species (ROS) generation in indomethacin-induced enteropathy, a well-known experimental model of Crohn''s disease. Seventy-eight male Wistar rats were randomly assigned to receive indomethacin, indomethacin + UDCA, or vehicles. Indomethacin induced a significant increase in the fraction of urinary excretion of 51Cr-EDTA following oral administration (7.9 ± 1.3 vs 2.3 ± 0.2%; P < 0.05) and lucigenin-amplified chemiluminescence in intestinal fragments ex vivo (10.1 ± 1.9 vs 2.6 ± 0.4 cpm × 103/mg; P < 0.05) compared to controls. UDCA significantly reversed these effects (P < 0.05), without being incorporated in biliary bile acid composition (HPLC analysis). These findings support a local protective effect of UDCA in experimental ileitis by the modulation of intestinal barrier dysfunction and oxidative stress. In short, they provide insights into mechanisms of action of UDCA in intestinal inflammation and a new perspective on the treatment of Crohn's disease. (AU)

• Processo FAPESP: 99/11758-2 - Lesao intestinal induzida por antiinflamatorio nao esteroide (aine): papel do acido ursodeoxicolico.
• Beneficiário: Carlos Felipe Bernardes Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado