| Texto completo | |
| Autor(es): |
Número total de Autores: 3
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| Afiliação do(s) autor(es): | [1] Unesp Univ Estadual Paulista, Inst Biol Sci, Dept Biochem & Microbiol, Lab Ind Microbiol, IBRC, BR-13506900 Rio Claro, SP - Brazil
[2] Unesp Univ Estadual Paulista, Inst Biol Sci, Dept Biochem & Microbiol, Lab Polymer Biotreatments, IBRC, BR-13506900 Rio Claro, SP - Brazil
Número total de Afiliações: 2
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| Tipo de documento: | Artigo Científico |
| Fonte: | Carbohydrate Polymers; v. 88, n. 4, p. 1440-1444, MAY 16 2012. |
| Citações Web of Science: | 34 |
| Resumo | |
This paper offers the physical and chemical characterization of a new dextran produced by Leuconostoc mesenteroides FT045B. The chemical structure was determined by Fourier Transform Infrared spectroscopy and H Nuclear Magnetic Resonance spectroscopy. The dextran was hydrolyzed by endodextranase; the products were analyzed using thin layer chromatography and compared with those of commercial B-512F dextran. The number-average molecular weight and degree of polymerization of the FT045B dextran were determined by the measurement of the reducing value using the copper bicinchoninate method and the measurement of total carbohydrate using the phenol-sulfuric acid method. The data revealed that the structure of the dextran synthesized by FT045B dextran sucrase is composed of D-glucose residues, containing 97.9% alpha-(1,6) linkages in the main chains and 2.1% alpha-(1,3) branch linkages compared with the commercial B-512F dextran, which has 95% alpha-(1,6) linkages in the main chains and 5% alpha-(1,3) branch linkages. (C) 2012 Elsevier Ltd. All rights reserved. (AU) | |
| Processo FAPESP: | 08/07410-1 - Estudo da produção de dextranasacarase por Leuconostoc mesenteroides FT 045B empregando planejamento experimental composto central |
| Beneficiário: | Mary Helen Palmuti Braga Vettori |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |