Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase

Author(s)

Palmuti Braga Vettori, Mary Helen ^[1] ; Martins Franchetti, Sandra Mara ^[2] ; Contiero, Jonas ^[1]

Affiliation

^[1] Unesp Univ Estadual Paulista, Inst Biol Sci, Dept Biochem & Microbiol, Lab Ind Microbiol, IBRC, BR-13506900 Rio Claro, SP - Brazil

^[2] Unesp Univ Estadual Paulista, Inst Biol Sci, Dept Biochem & Microbiol, Lab Polymer Biotreatments, IBRC, BR-13506900 Rio Claro, SP - Brazil

• Document type: Journal article
• Source: Carbohydrate Polymers; v. 88, n. 4, p. 1440-1444, MAY 16 2012.
• Web of Science Citations: 34

Abstract

This paper offers the physical and chemical characterization of a new dextran produced by Leuconostoc mesenteroides FT045B. The chemical structure was determined by Fourier Transform Infrared spectroscopy and H Nuclear Magnetic Resonance spectroscopy. The dextran was hydrolyzed by endodextranase; the products were analyzed using thin layer chromatography and compared with those of commercial B-512F dextran. The number-average molecular weight and degree of polymerization of the FT045B dextran were determined by the measurement of the reducing value using the copper bicinchoninate method and the measurement of total carbohydrate using the phenol-sulfuric acid method. The data revealed that the structure of the dextran synthesized by FT045B dextran sucrase is composed of D-glucose residues, containing 97.9% alpha-(1,6) linkages in the main chains and 2.1% alpha-(1,3) branch linkages compared with the commercial B-512F dextran, which has 95% alpha-(1,6) linkages in the main chains and 5% alpha-(1,3) branch linkages. (C) 2012 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 08/07410-1 - Study of dextransucrase production from Leuconostoc mesenteroides FT 045B employing central compound experimental design
• Grantee: Mary Helen Palmuti Braga Vettori
• Support Opportunities: Scholarships in Brazil - Master