Anaerobic benzene degradation under denitrifying conditions: Peptococcaceae as dominant benzene degraders and evidence for a syntrophic process

Autor(es)

van der Zaan, Bas M. ^[1] ; Saia, Flavia Talarico ^{[2, 3]} ; Stams, Alfons J. M. ^[2] ; Plugge, Caroline M. ^[2] ; de Vos, Willem M. ^[2] ; Smidt, Hauke ^[2] ; Langenhoff, Alette A. M. ^[1] ; Gerritse, Jan ^[1]

Afiliação do(s) autor(es)

^[1] Deltares, NL-3584 CB Utrecht - Netherlands

^[2] Wageningen Univ, Microbiol Lab, NL-6703 HB Wageningen - Netherlands

^[3] Univ Sao Paulo, Dept Hydraul & Sanitat, Engn Sch Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL MICROBIOLOGY; v. 14, n. 5, p. 1171-1181, MAY 2012.
• Citações Web of Science: 47

Resumo

An anaerobic microbial community was enriched in a chemostat that was operated for more than 8 years with benzene and nitrate as electron acceptor. The coexistence of multiple species in the chemostat and the presence of a biofilm, led to the hypothesis that benzene-degrading species coexist in a syntrophic interaction, and that benzene can be degraded in syntrophy by consortia with various electron acceptors in the same culture. The benzene-degrading microorganisms were identified by DNA-stable isotope probing with {[}U-13C]-labelled benzene, and the effect of different electron donors and acceptors on benzene degradation was investigated. The degradation rate constant of benzene with nitrate (0.7 day-1) was higher than reported previously. In the absence of nitrate, the microbial community was able to use sulfate, chlorate or ferric iron as electron acceptor. Bacteria belonging to the Peptococcaceae were identified as dominant benzene consumers, but also those related to Rhodocyclaceae and Burkholderiaceae were found to be associated with the anaerobic benzene degradation process. The benzene degradation activity in the chemostat was associated with microbial growth in biofilms. This, together with the inhibiting effect of hydrogen and the ability to degrade benzene with different electron acceptors, suggests that benzene was degraded via a syntrophic process. (AU)

• Processo FAPESP: 06/01997-5 - Utilização de 13C e da técnica do stable-isotope probing na identificação de metabólitos intermediários e de microrganismos degradadores de bezeno sob condições sulfato e nitrato-redutoras
• Beneficiário: Flávia Talarico Saia
• Modalidade de apoio: Bolsas no Exterior - Novas Fronteiras