MMP Activity in the Hybrid Layer Detected with in situ Zymography

Autor(es)

Mazzoni, A. ^{[1, 2, 3]} ; Nascimento, F. D. ^[4] ; Carrilho, M. ^[4] ; Tersariol, I. ^[5] ; Papa, V. ^[6] ; Tjaderhane, L. ^{[7, 8]} ; Di Lenarda, R. ^[1] ; Tay, F. R. ^[9] ; Pashley, D. H. ^[10] ; Breschi, L. ^{[1, 11]}

Afiliação do(s) autor(es):Mostrar menos -

^[1] Univ Trieste, Dept Med Sci, Unit Dent Sci & Biomat, I-34127 Trieste - Italy

^[2] Rizzoli Orthoped Inst, Cell Biol Lab, I-40136 Bologna - Italy

^[3] Rizzoli Orthoped Inst, Lab Immunorheumatol & Tissue Regenerat, Ramses Lab, I-40136 Bologna - Italy

^[4] Bandeirante Univ Sao Paulo UNIBANS, Biomat Res Grp, Sao Paulo - Brazil

^[5] Univ Mogi das Cruzes, Sao Paulo - Brazil

^[6] Univ Cassino, Dept Sport & Hlth Sci, Cassino - Italy

^[7] Univ Oulu, Inst Dent, Oulu - Finland

^[8] Oulu Univ Hosp, Oulu - Finland

^[9] Georgia Hlth Sci Univ, Coll Dent Med, Dept Endodont, Augusta, GA - USA

^[10] Georgia Hlth Sci Univ, Coll Dent Med, Dept Oral Biol, Augusta, GA - USA

^[11] IOR, Unit Bologna, IGM CNR, Bologna - Italy

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF DENTAL RESEARCH; v. 91, n. 5, p. 467-472, MAY 2012.
• Citações Web of Science: 78

Resumo

Dentinal proteases are believed to play an important role in the degradation of hybrid layers (HL). This study investigated the HL gelatinolytic activity by in situ zymography and functional enzyme activity assay. The hypotheses were that HLs created by an etch-and-rinse adhesive exhibit active gelatinolytic activity, and MMP-2 and -9 activities in dentin increase during adhesive procedures. Etched-dentin specimens were bonded with Adper Scotchbond 1XT and restored with composite. Adhesive/dentin interface slices were placed on microscope slides, covered with fluorescein-conjugated gelatin, and observed with a multi-photon confocal microscope after 24 hrs. Human dentin powder aliquots were prepared and assigned to the following treatments: A, untreated; B, etched with 10% phosphoric acid; or C, etched with 10% phosphoric acid and mixed with Scotchbond 1XT. The MMP-2 and -9 activities of extracts of dentin powder were measured with functional enzyme assays. Intense and continuous enzyme activity was detected at the bottom of the HL, while that activity was more irregular in the upper HL. Both acid-etching and subsequent adhesive application significantly increased MMP-2 and -9 activities (p < 0.05). The results demonstrate, for the first time, intrinsic MMP activity in the HL, and intense activation of matrix-bound MMP activity with both etching and adhesive application. (AU)

• Processo FAPESP: 11/12226-8 - Avaliação da atividade de proteases na degradação da dentina humana tratada por procedimentos restauradores adesivos
• Beneficiário: Marcela Rocha de Oliveira Carrilho
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 07/54618-4 - Avaliação da atividade de colagenases na degradação da dentina humana tratada por procedimentos restauradores adesivos
• Beneficiário: Marcela Rocha de Oliveira Carrilho
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores