Biomarkers for oxidative stress in acute lung injury induced in rabbits submitted to different strategies of mechanical ventilation

Autor(es)

Ronchi, Carlos Fernando ^{[1, 2]} ; Fioretto, Jose Roberto ^[3] ; Anjos Ferreira, Ana Lucia ^[2] ; Berchieri-Ronchi, Carolina Bragiola ^{[1, 2]} ; Correa, Camila Renata ^{[1, 2]} ; Kurokawa, Cilmery Suemi ^[3] ; Carpi, Mario Ferreira ^[3] ; Moraes, Marcos Aurelio ^[3] ; Yeum, Kyung-Jin ^[1]

Afiliação do(s) autor(es)

^[1] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 - USA

^[2] Sao Paulo State Univ UNESP, Dept Internal Med, Botucatu Med Sch, Botucatu, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Dept Pediat, Botucatu Med Sch, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Physiology; v. 112, n. 7, p. 1184-1190, APR 2012.
• Citações Web of Science: 7

Resumo

Ronchi CF, Fioretto JR, Ferreira ALA, Berchieri-Ronchi CB, Correa CR, Kurokawa CS, Carpi MF, Moraes MA, Yeum K-J. Biomarkers for oxidative stress in acute lung injury induced in rabbits submitted to different strategies of mechanical ventilation. J Appl Physiol 112: 1184-1190, 2012. First published February 2, 2012; doi:10.1152/japplphysiol.01334.2011.-Oxidative damage has been said to play an important role in pulmonary injury, which is associated with the development and progression of acute respiratory distress syndrome (ARDS). We aimed to identify biomarkers to determine the oxidative stress in an animal model of acute lung injury (ALI) using two different strategies of mechanical ventilation. Rabbits were ventilated using either conventional mechanical ventilation (CMV) or high-frequency oscillatory ventilation (HFOV). Lung injury was induced by tracheal saline infusion (30 ml/kg, 38 degrees C). In addition, five healthy rabbits were studied for oxidative stress. Isolated lymphocytes from peripheral blood and lung tissue samples were analyzed by alkaline single cell gel electrophoresis (comet assay) to determine DNA damage. Total antioxidant performance (TAP) assay was applied to measure overall antioxidant performance in plasma and lung tissue. HFOV rabbits had similar results to healthy animals, showing significantly higher antioxidant performance and lower DNA damage compared with CMV in lung tissue and plasma. Total antioxidant performance showed a significant positive correlation (r = 0.58; P = 0.0006) in plasma and lung tissue. In addition, comet assay presented a significant positive correlation (r = 0.66; P = 0.007) between cells recovered from target tissue and peripheral blood. Moreover, antioxidant performance was significantly and negatively correlated with DNA damage (r = -0.50; P = 0.002) in lung tissue. This study indicates that both TAP and comet assay identify increased oxidative stress in CMV rabbits compared with HFOV. Antioxidant performance analyzed by TAP and oxidative DNA damage by comet assay, both in plasma, reflects oxidative stress in the target tissue, which warrants further studies in humans. (AU)

• Processo FAPESP: 08/08199-2 - Efeito de diferentes técnicas de ventilação mecânica sobre o estresse oxidativo pulmonar induzido por modelo de síndrome do desconforto respiratório agudo em coelhos
• Beneficiário: Carlos Fernando Ronchi
• Modalidade de apoio: Bolsas no Brasil - Doutorado