Equid Herpesvirus Type-1 Exhibits Neurotropism and Neurovirulence in a Mouse Model

Autor(es)

Mori, C. M. C. ^[1] ; Mori, E. ^[2] ; Favaro, L. L. ^[2] ; Santos, C. R. ^[1] ; Lara, M. C. C. S. H. ^[3] ; Villalobos, E. M. C. ^[3] ; Cunha, E. M. S. ^[3] ; Brandao, P. E. ^[2] ; Richtzenhain, L. J. ^[2] ; Maiorka, P. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Pathol, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Prevent Vet Med & Anim Hlth, Sao Paulo - Brazil

^[3] Inst Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Comparative Pathology; v. 146, n. 2-3, p. 202-210, FEB-APR 2012.
• Citações Web of Science: 11

Resumo

Intranasal inoculation of equid herpesvirus type-1 (EHV-1) Brazilian strains A4/72 and A9/92 induced an acute and lethal infection in four different inbred mouse strains. Clinical and neurological signs appeared between the 2nd and 3rd day post inoculation (dpi) and included weight loss, ruffled fur, a hunched posture, crouching in corners, nasal and ocular discharges, dyspnoea, dehydration and increased salivation. These signs were followed by increased reactivity to external stimulation, seizures, recumbency and death. The virus was recovered consistently from the brain and viscera of all mice with neurological signs. Histopathological changes consisted of leptomeningitis, focal haemorrhage, ventriculitis, neuronal degeneration and necrosis, neuronophagia, non-suppurative inflammation, multifocal gliosis and perivascular infiltration of polymorphonuclear and mononuclear cells. Immunohistochemical examination demonstrated that EHV-1 strains A4/72 and A9/92 replicated in neurons of the olfactory bulb, the cortex and the hippocampus. In contrast, mice inoculated with the EHV-1 Brazilian strain A3/97 showed neither weight loss nor apparent clinical or neurological signs; however, the virus was recovered consistently from their lungs at 3 dpi. These three EHV-1 strains showed distinct degrees of virulence and tissue tropism in mice. EHV-1 strains A4/72 and A9/92 exhibited a high degree of central nervous system tropism with neuroinvasion and neurovirulence. EHV-1 strain A3/97 was not neurovirulent despite being detected in the brains of infected BALB/c nude mice. These findings indicate that several inbred mouse strains are susceptible to neuropathogenic EHV-1 strains and should be useful models for studying the pathogenesis and mechanisms contributing to EHV-induced myeloencephalopathy in horses. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 07/58861-0 - Caracterização sorológica e molecular da infecção viral em cavalos causada pelos herpesvírus equinos tipos 1 (EHV-1) e 4 (EHV-4)
• Beneficiário: Leonardo José Richtzenhain
• Modalidade de apoio: Auxílio à Pesquisa - Regular