The expression of antioxidant enzymes in the gingivae of type 2 diabetics with chronic periodontitis

Autor(es)

Duarte, Poliana M. ^[1] ; Napimoga, Marcelo H. ^[2] ; Fagnani, Ellen C. ^[3] ; Santos, Vanessa R. ^[1] ; Bastos, Marta F. ^[1] ; Ribeiro, Fernancla V. ^[1] ; Araujo, Vera C. ^[3] ; Demasi, Ana Paula D. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Guarulhos, Dent Res Div, Dept Periodontol, Sao Paulo - Brazil

^[2] Sao Leopoldo Mandic Inst & Res Ctr, Lab Immunol & Mol Biol, BR-13045755 Campinas, SP - Brazil

^[3] Sao Leopoldo Mandic Inst & Res Ctr, Pathol Lab, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF ORAL BIOLOGY; v. 57, n. 2, p. 161-168, FEB 2012.
• Citações Web of Science: 8

Resumo

Objectives: There is controversial evidence regarding the levels of antioxidant molecules in type 2 diabetes periodontitis patients. Thus, the aim of the present study was to evaluate the gene expression of antioxidant enzymes in the gingival tissue of poorly and well-controlled type 2 diabetic subjects with chronic periodontitis (CP). Design: Gingival biopsies were harvested from systemically and periodontally healthy subjects (n = 12), systemically healthy subjects with CP (n = 15), well-controlled (n = 8) and poorly controlled (n = 14) diabetic subjects with CP. The messenger RNA (mRNA) levels of peroxiredoxin (PRDX) 1 and 2, catalase (CAT), glutathione peroxidase (GPX1) and superoxide dismutase (SOD) 1 and 2 were measured by quantitative polymerase chain reaction (qPCR). Results: The results showed that PRDX1 and GPX1 were up-regulated by periodontitis (p < 0.05), independently of the glycaemic status, whilst PRDX2 and SOD2 genes were slightly influenced by periodontitis, but significantly induced when periodontitis was associated with DM, especially under a poor glycaemic control (p < 0.05). Moreover, CAT and SOD1 expressions were not significantly influenced by any of these inflammatory disorders (p > 0.05). Conclusion: In conclusion, both PRDX1 and GPX1 were overexpressed in CP whilst PRDX2 and SOD2 were up-regulated especially in the poorly controlled diabetic group with CP. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/04280-0 - Expressão gênica em sítios com periodontite crônica em indivíduos portadores de Diabetes Mellitus
• Beneficiário: Poliana Mendes Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/52200-8 - Estudo da função das peroxirredoxinas I e IV em células de mieloma múltiplo: avaliação de seu potencial como alvos terapêuticos
• Beneficiário: Ana Paula Dias Demasi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores