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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The expression of antioxidant enzymes in the gingivae of type 2 diabetics with chronic periodontitis

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Autor(es):
Duarte, Poliana M. [1] ; Napimoga, Marcelo H. [2] ; Fagnani, Ellen C. [3] ; Santos, Vanessa R. [1] ; Bastos, Marta F. [1] ; Ribeiro, Fernancla V. [1] ; Araujo, Vera C. [3] ; Demasi, Ana Paula D. [3]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Guarulhos, Dent Res Div, Dept Periodontol, Sao Paulo - Brazil
[2] Sao Leopoldo Mandic Inst & Res Ctr, Lab Immunol & Mol Biol, BR-13045755 Campinas, SP - Brazil
[3] Sao Leopoldo Mandic Inst & Res Ctr, Pathol Lab, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: ARCHIVES OF ORAL BIOLOGY; v. 57, n. 2, p. 161-168, FEB 2012.
Citações Web of Science: 8
Resumo

Objectives: There is controversial evidence regarding the levels of antioxidant molecules in type 2 diabetes periodontitis patients. Thus, the aim of the present study was to evaluate the gene expression of antioxidant enzymes in the gingival tissue of poorly and well-controlled type 2 diabetic subjects with chronic periodontitis (CP). Design: Gingival biopsies were harvested from systemically and periodontally healthy subjects (n = 12), systemically healthy subjects with CP (n = 15), well-controlled (n = 8) and poorly controlled (n = 14) diabetic subjects with CP. The messenger RNA (mRNA) levels of peroxiredoxin (PRDX) 1 and 2, catalase (CAT), glutathione peroxidase (GPX1) and superoxide dismutase (SOD) 1 and 2 were measured by quantitative polymerase chain reaction (qPCR). Results: The results showed that PRDX1 and GPX1 were up-regulated by periodontitis (p < 0.05), independently of the glycaemic status, whilst PRDX2 and SOD2 genes were slightly influenced by periodontitis, but significantly induced when periodontitis was associated with DM, especially under a poor glycaemic control (p < 0.05). Moreover, CAT and SOD1 expressions were not significantly influenced by any of these inflammatory disorders (p > 0.05). Conclusion: In conclusion, both PRDX1 and GPX1 were overexpressed in CP whilst PRDX2 and SOD2 were up-regulated especially in the poorly controlled diabetic group with CP. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

Processo FAPESP: 08/04280-0 - Expressão gênica em sítios com periodontite crônica em indivíduos portadores de Diabetes Mellitus
Beneficiário:Poliana Mendes Duarte
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 09/52200-8 - Estudo da função das peroxirredoxinas I e IV em células de mieloma múltiplo: avaliação de seu potencial como alvos terapêuticos
Beneficiário:Ana Paula Dias Demasi
Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores