Clustering Behavior in Microbial Communities from Acute Endodontic Infections

Autor(es)

Montagner, Francisco ^{[1, 2]} ; Jacinto, Rogerio C. ^{[2, 3]} ; Signoretti, Fernanda G. C. ^[2] ; Sanches, Paula F. ^[4] ; Gomes, Brenda P. F. A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Rio Grande do Sul, Endodont Div, Porto Alegre, RS - Brazil

^[2] Univ Estadual Campinas, Endodont Div, Dept Restorat Dent, Sao Paulo - Brazil

^[3] Univ Fed Pelotas, Endodont Div, Pelotas, RS - Brazil

^[4] Salesian Univ, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 38, n. 2, p. 158-162, FEB 2012.
• Citações Web of Science: 21

Resumo

Introduction: Acute endodontic infections harbor heterogeneous microbial communities in both the root canal (RC) system and apical tissues. Data comparing the microbial structure and diversity in endodontic infections in related ecosystems, such as RC with necrotic pulp and acute apical abscess (AAA), are scarce in the literature. The aim of this study was to examine the presence of selected endodontic pathogens in paired samples from necrotic RC and AAA using polymerase chain reaction (PCR) followed by the construction of cluster profiles. Methods: Paired samples of RC and AAA exudates were collected from 20 subjects and analyzed by PCR for the presence of selected strict and facultative anaerobic strains. The frequency of species was compared between the RC and the AAA samples. A stringent neighboring clustering algorithm was applied to investigate the existence of similar high-order groups of samples. A dendrogram was constructed to show the arrangement of the sample groups produced by the hierarchical clustering. Results: All samples harbored bacterial DNA. Porphyromonas endodontalis, Prevotella nigrescens, Filifactor alocis, and Tannerela forsythia were frequently detected in both RC and AAA samples. The selected anaerobic species were distributed in diverse small bacteria consortia. The samples of RC and AAA that presented at least one of the targeted microorganisms were grouped in small clusters. Conclusions: Anaerobic species were frequently detected in acute endodontic infections and heterogeneous microbial communities with low clustering behavior were observed in paired samples of RC and AAA. (J Endod 2012;38:158-162) (AU)

• Processo FAPESP: 08/57551-0 - Microbiota dos canais radiculares de dentes com insucesso do tratamento endodôntico e análise da suscetibilidade antimicrobiana e dos fatores de virulência dos Enterococcus faecalis isolados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51113-1 - Efetividade in vitro de soluções irrigadoras e medicações intracanais sobre microrganismos e endotoxina de canais radiculares
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 07/58518-4 - Análise da microbiota de canais radiculares de dentes humanos infectados através da cultura, clonagem e sequenciamento do gene 16S rRNA
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57954-8 - Estudo microbiológico, quantificação de endotoxinas e investigação da atividade endotóxica de diferentes LPS bacterianos isolados de canais radiculares de dentes com necrose pulpar e presença de lesão periapiacal
• Beneficiário: Frederico Canato Martinho
• Modalidade de apoio: Bolsas no Brasil - Doutorado