In vitro influence of the extracellular matrix in myoepithelial cells stimulated by malignant conditioned medium

Autor(es)

Martinez, Elizabeth F. ^[1] ; Dias Demasi, Ana Paula ^[1] ; Napimoga, Marcelo Henrique ^[1] ; Arana-Chavez, Victor Elias ^[2] ; Altemani, Albina ^[3] ; de Araujo, Ney Soares ^[1] ; de Araujo, Vera Cavalcanti ^[1]

Afiliação do(s) autor(es)

^[1] Sao Leopoldo Mand Inst & Res Ctr, Dept Oral Pathol, BR-13045610 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent, Lab Oral Biol, Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Dept Pathol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Oral Oncology; v. 48, n. 2, p. 102-109, FEB 2012.
• Citações Web of Science: 9

Resumo

In order to investigate the role of myoepithelial cell and tumor microenvironment in salivary gland neoplasma, we have performed a study towards the effect of different extracellular matrix proteins (basement membrane matrix, type I collagen and fibronectin) on morphology and differentiation of benign myoepithelial cells from pleomorphic adenoma cultured with malignant cell culture medium from squamous cell carcinoma. We have also analyzed the expression of alpha-smooth muscle actin (alpha-SMA) and FGF-2 by immunofluorescence and qPCR. Our immunofluorescence results, supported by qPCR analysis, demonstrated that alpha-SMA and FGF-2 were upregulated in the benign myoepithelial cells from pleomorphic adenoma in all studied conditions on fibronectin substratum. However, the myoepithelial cells on fibronectin substratum did not alter their morphology under malignant conditioned medium stimulation and exhibited a stellate morphology and, occasionally focal adhesions with the substratum. In summary, our data demonstrated that the extracellular matrix exerts an important role in the morphology of the benign myoepithelial cells by the presence of focal adhesions and also inducing increase FGF-2 and alpha-SMA expression by these cells, especially in the fibronectin substratum. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/58721-7 - Alterações morfológicas e fenotípicas das células mioepiteliais estimuladas por células de carcinoma
• Beneficiário: Elizabeth Ferreira Martinez
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 08/58722-3 - Alterações morfológicas e fenotípicas das células mioepiteliais estimuladas por células de carcinoma
• Beneficiário: Vera Cavalcanti de Araujo
• Modalidade de apoio: Auxílio à Pesquisa - Regular