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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro influence of the extracellular matrix in myoepithelial cells stimulated by malignant conditioned medium

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Author(s):
Martinez, Elizabeth F. [1] ; Dias Demasi, Ana Paula [1] ; Napimoga, Marcelo Henrique [1] ; Arana-Chavez, Victor Elias [2] ; Altemani, Albina [3] ; de Araujo, Ney Soares [1] ; de Araujo, Vera Cavalcanti [1]
Total Authors: 7
Affiliation:
[1] Sao Leopoldo Mand Inst & Res Ctr, Dept Oral Pathol, BR-13045610 Campinas, SP - Brazil
[2] Univ Sao Paulo, Sch Dent, Lab Oral Biol, Sao Paulo - Brazil
[3] Univ Estadual Campinas, Dept Pathol, Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Oral Oncology; v. 48, n. 2, p. 102-109, FEB 2012.
Web of Science Citations: 9
Abstract

In order to investigate the role of myoepithelial cell and tumor microenvironment in salivary gland neoplasma, we have performed a study towards the effect of different extracellular matrix proteins (basement membrane matrix, type I collagen and fibronectin) on morphology and differentiation of benign myoepithelial cells from pleomorphic adenoma cultured with malignant cell culture medium from squamous cell carcinoma. We have also analyzed the expression of alpha-smooth muscle actin (alpha-SMA) and FGF-2 by immunofluorescence and qPCR. Our immunofluorescence results, supported by qPCR analysis, demonstrated that alpha-SMA and FGF-2 were upregulated in the benign myoepithelial cells from pleomorphic adenoma in all studied conditions on fibronectin substratum. However, the myoepithelial cells on fibronectin substratum did not alter their morphology under malignant conditioned medium stimulation and exhibited a stellate morphology and, occasionally focal adhesions with the substratum. In summary, our data demonstrated that the extracellular matrix exerts an important role in the morphology of the benign myoepithelial cells by the presence of focal adhesions and also inducing increase FGF-2 and alpha-SMA expression by these cells, especially in the fibronectin substratum. (C) 2011 Elsevier Ltd. All rights reserved. (AU)