In vitro evaluation of adhesion molecules expression on in situ like neoplastic malignant areas under tumoral microenvironment influence.

• Grant number: 11/19082-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2012
• End date: January 31, 2013
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Elizabeth Ferreira Martinez
• Grantee: Natalia Festugatto Navarini
• Host Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Campinas , SP, Brazil

Abstract

The tumor microenvironment, during the development of neoplastic process, has been studied, especially in breast cancer, and is considered a determinant factor in the behavior of cancer. Among all the components involved in the tumor microenvironment, the stromal cells represented by the myoepithelial cells in gland tumors have an important role. Pleomorphic adenoma awake interest in the study of tumors, for being the most common tumor gland and one of the few benign neoplasms that can undergo malignant transformation, sometimes mediated by myoepithelial cells. Besides, the extracellular matrix and growth factors can regulate the proliferation and differentiation of these cells contributing to metastasis. Thus, the objective of this study is to evaluate cell-cell and cell-matrix interactions in areas that represent a situation when in situ squamous cell carcinoma of the malignant cells are surrounded by benign myoepithelial cells of pleomorphic adenoma, under the influence of an extracellular matrix protein , collagen type I and epidermal growth factor (EGF), and thus simulating the condition of the tumor microenvironment at different times, in order to identify the moment and presence of expression of these proteins in the neoplastic process. It will be assessed by indirect immunofluorescence the immunoexpression of ±2²1 integrin protein, E-cadherin and ²-catenin in the areas that simulate the in situ condition.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

NAVARINI, NATALIA FESTUGATTO; DE ARAUJO, VERA CAVALCANTI; BROWN, AMY LOUISE; PASSADOR-SANTOS, FABRICIO; DE SOUZA, ISABELA FERNANDES; NAPIMOGA, MARCELO HENRIQUE; ARAUJO, NEY SOARES; MARTINEZ, ELIZABETH FERREIRA. The EGF signaling pathway influences cell migration and the secretion of metalloproteinases by myoepithelial cells in pleomorphic adenoma. TUMOR BIOLOGY, v. 36, n. 1, p. 205-211, JAN 2015. (11/19082-1)