Influence of low-level laser associated with osteogenic proteins recombinant human BMP-2 and Hevea brasiliensis on bone repair in Wistar rats

Autor(es)

Iyomasa, Mamie Mizusaki ^[1] ; Mardegan Issa, Joao Paulo ^[1] ; de Queiroz Tavares, Mirella Lemos ^[1] ; Lara Pereira, Yamba Carla ^[1] ; Sasso Stuani, Maria Bernadete ^[2] ; Mishima, Fabiola ^[2] ; Coutinho-Netto, Joaquim ^[3] ; Sebald, Walter ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Morfol Estomatol & Fisiol, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Clin Infantil Odontol Prevent & Social, BR-14040904 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Univ Wurzburg, Theodor Boveri Inst Biowissensch, Wurzburg - Germany

• Tipo de documento: Artigo Científico
• Fonte: MICROSCOPY RESEARCH AND TECHNIQUE; v. 75, n. 2, p. 117-125, FEB 2012.
• Citações Web of Science: 7

Resumo

This study analyzed the newly formed bone tissue after application of recombinant human BMP-2 (rhBMP-2) and P-1 (extracted from Hevea brasiliensis) proteins, 2 weeks after the creation of a critical bone defect in male Wistar rats treated or not with a low-intensity laser (GaAlAs 780 nm, 60 mW of power, and energy density dose of 30 J/cm2). The animals were divided into two major groups: (1) bone defect plus low-intensity laser treatment and (2) bone defect without laser irradiation. The following subgroups were also analyzed: (a) 5 mu g of pure rhBMP-2; (b) 5 mu g of pure P-1 fraction; (c) 5 mu g of rhBMP-2/monoolein gel; (d) 5 mu g of P-1 fraction/monoolein gel; (e) pure monoolein gel. Comparisons of the groups receiving laser treatment with those that did not receive laser irradiation show differences in the areas of new bone tissue. The group treated with 5 mu g of rhBMP-2 and laser irradiation was not significantly different (P >0.05) than the nonirradiated group that received the same treatment. The irradiated, rhBMP-2/monoolein gel treatment group showed a lower area of bone formation than the nonirradiated, rhBMP-2/gel monoolein treatment group (P < 0.001). The area of new bone tissue in the other nonirradiated and irradiated groups was not significantly different (P > 0.05). Furthermore, the group that received the 5 mu g of rhBMP-2 application showed the greatest bone formation. We conclude that the laser treatment did not interfere with the area of new bone tissue growth and that the greatest stimulus for bone formation involved application of the rhBMP-2 protein. Microsc. Res. Tech. 2011. (c) 2011 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 08/55486-7 - Influência do laser de baixa intensidade associado as proteínas osteogênicas rhBMP-2 e Hevea brasiliensis no reparo de defeitos ósseos em calvária de ratos Wistar
• Beneficiário: Mamie Mizusaki Iyomasa
• Modalidade de apoio: Auxílio à Pesquisa - Regular