| Texto completo | |
| Autor(es): |
Silva, Synara Araujo
[1]
;
Rodrigues, Celia Lima
[1, 2]
;
Campos, Aleia Faustino
[1]
;
Levi, Jose Eduardo
[1, 2]
Número total de Autores: 4
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Med, Dept Infect Dis, BR-05403000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Trop Med, Virol Lab, BR-05403000 Sao Paulo - Brazil
Número total de Afiliações: 2
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Revista do Instituto de Medicina Tropical de São Paulo; v. 54, n. 1, p. 31-35, JAN-FEB 2012. |
| Citações Web of Science: | 2 |
| Resumo | |
The present study aimed at standardizing a real-time quantitative polymerase chain reaction assay to evaluate the presence of GBV-C/HGV RNA. A ``TaqMan{''} assay using primers and probe derived from the 5' NCR region was developed and validated. Two hundred and fifty-three plasma samples from HIV-infected women were tested for GBV-C viremia and antibody against the envelope protein 2. GBV-C RNA was detected in 22.5% of the patients whereas the antibody was identified in 25.3% of the cohort. Detection of viral RNA and of antibodies was mutually exclusive. Viral loads showed a mean of 1,777 arbitrary units/mL, being 1.1 and 13,625 arbitrary units/mL respectively the lowest and highest values measured. We conclude that the real-time quantitative polymerase chain reaction method developed is appropriate for the investigation of GBV-C RNA since it was shown to be highly specific and sensitive, as well as requiring few steps, preventing contamination and providing additional information as to the relative viremia of carriers, a parameter that must be included in studies evaluating the co-factors influencing the clinical outcome of HIV/AIDS. (AU) | |
| Processo FAPESP: | 05/01072-9 - A influência da infecção pelo GB virus com virus da hepatite G (HGV) na evolução da doença pelo HIV em mulheres |
| Beneficiário: | José Eduardo Levi |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |