Conformational stability of recombinant manganese superoxide dismutase from the filamentous fungus Trichoderma reesei

Autor(es)

Chambergo, Felipe S. ^[1] ; Valencia, Estela Y. ^[2] ; Ferreira-Junior, Jose Ribamar ^[1] ; Camilo, Cesar M. ^[3] ; Campana, Patricia T. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, BR-03828000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biociencias, BR-05513970 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 50, n. 1, p. 19-24, JAN 1 2012.
• Citações Web of Science: 3

Resumo

Superoxide dismutases (SODS; EC 1.15.1.1) are part of the antioxidant system of aerobic organisms and are used as a defense against oxidative injury caused by reactive oxygen species (ROS). The cloning and sequencing of the 788-bp genomic DNA from Trichoderma reesei strain QM9414 (anamorph of Hypocrea jecorina) revealed an open reading frame encoding a protein of 212 amino acid residues, with 65-90% similarity to manganese superoxide dismutase from other filamentous fungi. The TrMnSOD was purified and shown to be stable from 20 to 90 degrees C for 1 h at pH from 8 to 11.5, while maintaining its biological activity. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 07/50567-6 - Metabolismo de compostos aromáticos em Trichoderma reesei
• Beneficiário: Felipe Santiago Chambergo Alcalde
• Modalidade de apoio: Auxílio à Pesquisa - Regular