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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Conformational stability of recombinant manganese superoxide dismutase from the filamentous fungus Trichoderma reesei

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Author(s):
Chambergo, Felipe S. [1] ; Valencia, Estela Y. [2] ; Ferreira-Junior, Jose Ribamar [1] ; Camilo, Cesar M. [3] ; Campana, Patricia T. [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, BR-03828000 Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biociencias, BR-05513970 Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 50, n. 1, p. 19-24, JAN 1 2012.
Web of Science Citations: 3
Abstract

Superoxide dismutases (SODS; EC 1.15.1.1) are part of the antioxidant system of aerobic organisms and are used as a defense against oxidative injury caused by reactive oxygen species (ROS). The cloning and sequencing of the 788-bp genomic DNA from Trichoderma reesei strain QM9414 (anamorph of Hypocrea jecorina) revealed an open reading frame encoding a protein of 212 amino acid residues, with 65-90% similarity to manganese superoxide dismutase from other filamentous fungi. The TrMnSOD was purified and shown to be stable from 20 to 90 degrees C for 1 h at pH from 8 to 11.5, while maintaining its biological activity. (C) 2011 Elsevier B.V. All rights reserved. (AU)