Tributyltin in crustacean tissues: analytical performance and validation of method

Autor(es)

Cristale, Joyce ^[1] ; dos Santos, Dayana M. ^[2] ; Sant'Anna, Bruno S. ^[3] ; Sandron, Daniela C. ^[1] ; Cardoso, Sara ^[1] ; Turra, Alexander ^[4] ; de Marchi, Mary Rosa R. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Analyt Chem, Inst Chem, BR-14800900 Araraquara, SP - Brazil

^[2] Univ Sao Paulo, Dept Chem & Geol Oceanog, Oceanog Inst, BR-05508900 Sao Paulo - Brazil

^[3] Sao Paulo State Univ UNESP, Dept Zool, Inst Biosci, BR-13506900 Rio Claro, SP - Brazil

^[4] Univ Sao Paulo, Dept Biol Oceanog, Oceanog Inst, BR-05508120 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of the Brazilian Chemical Society; v. 23, n. 1, p. 39-45, JAN 2012.
• Citações Web of Science: 7

Resumo

The hermit crab Clibanarius vittatus is a typical organism from intertidal regions being considered as a good bioindicator of tributyltin presence at these environments. Thus this study presents the analytical performance and validation method for TBT quantification in tissues of C. vittatus by gas chromatography with pulsed flame photometric detector (GC-PFPD) after extraction with an apolar solvent (toluene) and Grignard derivatization. The limits of detection of the method (LOD) were 2.0 and 2.8 ng g-1 for TBT and DBT (dibutyltin), respectively, and its limits of quantification (LOQ) were 6.6 and 8.9 ng g-1 for TBT and DBT, respectively. The method was applied to samples from Santos Estuary, São Paulo State, Brazil. TBT and DBT concentrations ranged from 26.7 to 175.0 ng g-1 and from 46.2 to 156.0 ng g-1, respectively. These concentrations are worrisome since toxic effects (such as endocrine disruption) have been reported for other organisms even under lower levels of registred at this study. (AU)

• Processo FAPESP: 06/57007-3 - Avaliação da contaminação do ermitão Clibanarius vittatus (Decapoda, Anomura) por TBT (tributilestanho) na natureza e determinação experimental de sua via de assimilação, tempo de depuração e efeito na intersexualidade
• Beneficiário: Alexander Turra
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 06/61589-8 - Análise experimental da contaminação do ermitão Clibanarius vittatus (Decapoda, Anomura) por TBT (tributilestanho): em busca de uma resposta para a intersexualidade em ermitões
• Beneficiário: Bruno Sampaio Sant'Anna
• Modalidade de apoio: Bolsas no Brasil - Doutorado