Effectiveness of Photodynamic Therapy for the Inactivation of Candida spp. on Dentures: In Vitro Study

Autor(es)

de Oliveira Mima, Ewerton Garcia ^[1] ; Pavarina, Ana Claudia ^[2] ; Ribeiro, Daniela Garcia ^[3] ; Dovigo, Livia Nordi ^[2] ; Vergani, Carlos Eduardo ^[2] ; Bagnato, Vanderlei Salvador ^[4]

Afiliação do(s) autor(es)

^[1] Univ Sao Francisco, Sch Dent, BR-12900000 Braganca Paulista, SP - Brazil

^[2] UNESP Univ Estadual Paulista, Dept Dent Mat & Prosthodont, Araraquara Dent Sch, Sao Paulo - Brazil

^[3] Ponta Grossa State Univ UEPG, Dept Dent, Ponta Grossa, PR - Brazil

^[4] Univ Sao Paulo, Inst Phys, Carlos - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photomedicine and Laser Surgery; v. 29, n. 12, p. 827-833, DEC 2011.
• Citações Web of Science: 23

Resumo

Objective: This in vitro study evaluated the effectiveness of photodynamic therapy (PDT) for the inactivation of different species of Candida on maxillary complete dentures. Background data: The treatment of denture stomatitis requires the inactivation of Candida spp. on dentures. PDT has been reported as an effective method for Candida inactivation. Methods: Reference strains of C. albicans, C. glabrata, C. tropicalis, C. dubliniensis and C. krusei were tested. Thirty-four dentures were fabricated in a standardized procedure and subjected to ethylene oxide sterilization. The dentures were individually inoculated with one of the strains and incubated at 37 degrees C for 24 h. Dentures submitted to PDT (P + L +) were individually sprayed with 50 mg/L of Photogem (R) (PS) and, after 30 min, illuminated by LED light for 26 min (37.5 J/cm(2)). Additional dentures were treated only with PS (P + L-) or light (P-L +) or neither (P-L-). Samples of serial dilutions were spread on Sabouraud dextrose agar and incubated at 37 degrees C for 48 h. The colonies were counted and the values of log (cfu/mL) were analyzed by Kruskall-Wallis and Dunn tests (p < 0.05). Results: For all species of Candida, PDT resulted in significant reduction (p < 0.05) of cfu/mL values from dentures when compared with P-L-(reductions from 1.73 to 3.99 log(10)). Significant differences (p < 0.05), but lower reductions, were also observed for P + L-and P-L + when compared with P-L-for some species of Candida. Conclusions: PDT was an effective method for reducing Candida spp. on dentures. (AU)

• Processo FAPESP: 05/03226-3 - Estudo da viabilidade da utilizacao da terapia fotodinamica no controle da estomatite protetica.
• Beneficiário: Ana Cláudia Pavarina
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/02193-4 - Estudo da viabilidade da utilização da terapia fotodinâmica no controle da estomatite protética
• Beneficiário: Ewerton Garcia de Oliveira Mima
• Modalidade de apoio: Bolsas no Brasil - Doutorado