In vitro conservation and low cost micropropagation of Cochlospermum regium (Mart. Ex. Scharank)

Autor(es)

Inacio, Marielle Cascaes ^{[1, 2]} ; Bertoni, Bianca Waleria ^[1] ; Franca, Suzelei de Castro ^[1] ; Soares Pereira, Ana Maria ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Ribeirao Preto, Unidade Biotecnol, BR-14096900 Ribeirao Preto - Brazil

^[2] Univ Estadual Paulista, Programa Posgrad Agron Hort, Fac Ciencias Agron, BR-18610307 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Medicinal Plants Research; v. 5, n. 20, p. 4999-5007, SEP 30 2011.
• Citações Web of Science: 3

Resumo

Cochlospermum regium (Mart. Ex. Scharank), a Cochlospermaceae is a Cerrado medicinal species showing antimicrobial activity on the female reproductive system. The species risks being genetically eroded since it is highly explored, the biome devastated and the plant roots are employed for phytotherapeutic preparations. This study aims to develop a micropropagation protocol for large scale production of plantlets and establish the species conservation in a germplasm bank, in vitro. Nodal segments were inoculated into Murashige and Skoog (MS) medium supplemented with different concentrations of sucrose, BAP, kinetin, zeatin, Phytagel (R) and activated charcoal. The bud position effect on the multiplication index was evaluated and the auxin IBA utilized in rooting experiments. The best multiplication response was with the apical bud in culture medium in absence of cytokinins. MS inoculated explants with different IBA concentrations did not root in vitro, but ex vitro rooting was satisfactory. Survival of explants, 47.62%, was for 4 months in germplasm bank conditions without culture medium transference. (AU)

• Processo FAPESP: 08/52720-9 - Conservação in vitro de Cochlospermum regium (Mart. Ex. Scharank.): uma planta medicinal do cerrado
• Beneficiário: Ana Maria Soares Pereira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/52719-0 - Conservação in vitro de Cochlospermum regium (Mart. Ex. Scharank.): uma planta medicinal do cerrado
• Beneficiário: Marielle Cascaes Inácio
• Modalidade de apoio: Bolsas no Brasil - Mestrado