Sexing single bovine blastomeres using TSPY gene amplification

Autor(es)

Carneiro, M. C. A. ^{[1, 2]} ; Takeuchi, P. L. ^[1] ; Araujo, A. ^[1] ; Lobo, R. B. ^[1] ; Elias, F. P. ^[1] ; Vila, R. A. ^[1] ; Miranda-Furtado, C. L. ^[1] ; Ramos, E. S. ^{[3, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14049 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Ginecol & Obstet, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Genetics and Molecular Research; v. 10, n. 4, p. 3937-3941, 2011.
• Citações Web of Science: 4

Resumo

The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each embryo. A total of 36 single blastomeres and the remaining cells of their 18 matched in vitro conceived embryos were screened for TSPY amplification by nested-PCR. The results obtained from a single blastomere and the remaining cells of the same embryo were concordant in all cases. All blastomeres (16/16) from eight embryos produced with sexed sperm (specific for production of male embryos) were TSPY-positive. We conclude that TSPY is a good male-specific marker, the usefulness of which is probably enhanced by the high copy number. Other methods that are less time-consuming, such as real-time PCR, could be improved with the use of the TSPY gene sequences to generate primers and/or probes. This is the first report to demonstrate the applicability of the TSPY gene for sexing single cells in cattle. (AU)

• Processo FAPESP: 09/08313-2 - Expressão diferencial e padrão de metilação da região MHM durante o desenvolvimento gonadal de galinhas
• Beneficiário: Ester Silveira Ramos
• Modalidade de apoio: Auxílio à Pesquisa - Regular