| Full text | |
| Author(s): |
Carneiro, M. C. A.
[1, 2]
;
Takeuchi, P. L.
[1]
;
Araujo, A.
[1]
;
Lobo, R. B.
[1]
;
Elias, F. P.
[1]
;
Vila, R. A.
[1]
;
Miranda-Furtado, C. L.
[1]
;
Ramos, E. S.
[3, 1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Genet, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14049 Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Ginecol & Obstet, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Genetics and Molecular Research; v. 10, n. 4, p. 3937-3941, 2011. |
| Web of Science Citations: | 4 |
| Abstract | |
The testis-specific protein Y-encoded gene (TSPY) is a Y-specific gene present in variable copy number in many mammalian species, including cattle. We tested the applicability of the TSPY gene as a Y-specific marker to predict preimplantation embryo sex in Nelore (Bos indicus) cattle. Two blastomeres were removed from each embryo. A total of 36 single blastomeres and the remaining cells of their 18 matched in vitro conceived embryos were screened for TSPY amplification by nested-PCR. The results obtained from a single blastomere and the remaining cells of the same embryo were concordant in all cases. All blastomeres (16/16) from eight embryos produced with sexed sperm (specific for production of male embryos) were TSPY-positive. We conclude that TSPY is a good male-specific marker, the usefulness of which is probably enhanced by the high copy number. Other methods that are less time-consuming, such as real-time PCR, could be improved with the use of the TSPY gene sequences to generate primers and/or probes. This is the first report to demonstrate the applicability of the TSPY gene for sexing single cells in cattle. (AU) | |
| FAPESP's process: | 09/08313-2 - Methylation pattern and differential MHM region expression during chicken gonadal development |
| Grantee: | Ester Silveira Ramos |
| Support Opportunities: | Regular Research Grants |