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Dwarf brocket deer (Mazama nana) distribution and population estimates using non-invasive genetics sampling and modeling

Abstract

The dwarf brocket deer (Mazaman nana) is a cervid species that occupies the forests of Southern Brazil and has been greatly affected by the drastic reduction of forested areas. It is also the neotropical deer less studied by Science. Aware about this situation, this project aims to determine the species basic ecological parameters such as distribution, abundance and sex ratio. Given the rarity and high elusiveness of the species, it is proposed the use of indirect methods to achieve this objective. Thus, will be used methodologies based on the collect of faecal samples, DNA extraction and subsequent molecular and genetic analysis. Faecal samples will be collected with the help from a scat detection dog, in protected areas spread over the south of Brazil. After species identification by PCR/RFLP and specialization of the samples, will be made the species distribution modeling at the software Maxent using the environmental variables from Ambdata database of INPE. A protected area will be choose for a faecal sampling based on transects, every month for three months, in order to estimate the population through models of capture-mark-recapture. With this data will be calculated the density of the species in the area sampled. For this faeces samples will be individualized with microsatellite markers. The sex ratio will be calculated with the abundance data and the sexing of the samples by the amelogenin locus amplification. This information will contribute to the beginning of an adequate species monitoring program and to determine priority areas for the dwarf brocket deer conservation. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PIRES TEIXEIRA, WESLEN FABRICIO; DE OLIVEIRA, MARCIO LEITE; DE FARIA PERES, PEDRO HENRIQUE; MIRANDA OLIVEIRA, BRUNO CESAR; NAGATA, WALTER BERTEQUINI; VIEIRA, DIELSON DA SILVA; DE ANDRADE JUNIOR, ANASSILTON MOREIRA; FERRARI, ELIS DOMINGOS; BARBANTI DUARTE, JOSE MAURICIO; MEIRELES, MARCELO VASCONCELOS; et al. IRST REPORT OF GENUS Cryptosporidium IN CERVIDS SPECIES: Mazama americana, Mazama nana AND Blastocerus dichotomu. VETERINARY RESEARCH COMMUNICATIONS, v. 46, n. 1, . (10/50748-3, 15/10086-5, 12/50206-1, 15/25742-5)
PIRES TEIXEIRA, WESLEN FABRICIO; DE OLIVEIRA, MARCIO LEITE; DE FARIA PERES, PEDRO HENRIQUE; NAGATA, WALTER BERTEQUINI; SANTANA, BRUNA NICOLETI; MIRANDA OLIVEIRA, BRUNO CESAR; BARBANTI DUARTE, JOSE MAURICIO; CARDOSO, TEREZA CRISTINA; ZANETTI LOPES, WELBER DANIEL; SARAIVA BRESCIANI, KATIA DENISE. Cryptosporidium parvum in brown brocket (Mazama gouazoubira) from Brazil: First report of the subtype IIaA16G3R1 in cervids. Parasitology International, v. 80, . (10/50748-3, 15/10086-5, 15/25742-5, 12/50206-1)
DE OLIVEIRA, MARCIO LEITE; ZARATE DO COUTO, HILTON THADEU; BARBANTI DUARTE, JOSE MAURICIO. Distribution of the elusive and threatened Brazilian dwarf brocket deer refined by non-invasive genetic sampling and distribution modelling. EUROPEAN JOURNAL OF WILDLIFE RESEARCH, v. 65, n. 2, . (12/01095-2, 12/50206-1, 15/25742-5)