The extrinsic apoptotic signaling pathway in gastric adenocarcinomas assessed by tissue microarray

Autor(es)

Gomes, Thiago S. ^{[1, 2]} ; Oshima, Celina T. F. ^{[1, 2]} ; Segreto, Helena R. C. ^[3] ; Barrazueta, Luis M. ^[1] ; Costa, Henrique O. ^[1] ; Lima, Flavio O. ^[1] ; Forones, Nora M. ^[2] ; Ribeiro, Daniel A. ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, UNIFESP, Dept Patol, Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, UNIFESP, Grp Oncol, Div Gastroentererol, Dept Med, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, UNIFESP, Lab Radiol, Dept Med, Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo, Dept Biociencias, UNIFESP, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PATHOLOGY RESEARCH AND PRACTICE; v. 207, n. 10, p. 613-617, 2011.
• Citações Web of Science: 5

Resumo

The purpose of this investigation was to analyze the immunoexpression of FasL, Fas, FADD, cleaved caspase 8, and cleaved caspase 3 in gastric cancer. Formalin-fixed and paraffin-embedded gastric adenocarcinoma tissues from 87 patients, including adjacent normal tissues, were included on tissue microarray by immunohistochemistry. The tumor and the adjacent normal tissues were positive for FasL in 66.7% and 90.6%, for Fas in 52.8% and 52.4%, for FADD in 67.4% and 82.3%, for cleaved caspase 8 in 27.9% and 37.7%, and for cleaved caspase 3 in 33.7% and 8.3%, respectively. FasL and the FADD from tumor were statistically different in relation to the histological type. Cleaved caspase 8 was statistically different in relation to clinical stage (p = 0.031). The FADD from normal tissue was statistically different in relation to age (p = 0.039), sex (p = 0.055), clinical stage (p = 0.019), and Fas was different in relation to tumor size (p = 0.012). In the tumor, we observed a correlation between FasL and Fas, FasL and FADD, and FasL and cleaved caspase 3. In the adjacent normal tissue, a correlation was observed between FasL and Fas, FasL and FADD. There was no association of another marker with sex, age, clinical stage, and survival. Our results suggest that these proteins mediate the early extrinsic apoptotic pathway in gastric cancer and adjacent normal mucosa. FasL protein binds to Fas protein and subsequently binds to death receptor FADD signaling activation of the extrinsic apoptotic pathway. In this phase, there was inhibition of caspase 8 and, consequently, decreased apoptosis. (C) 2011 Elsevier GmbH. All rights reserved. (AU)

• Processo FAPESP: 04/09932-4 - Apoptose em tumores
• Beneficiário: Marcello Fabiano de Franco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 06/54187-0 - Imunoexpressao de proteinas reguladoras de apoptose bcl 2 e bax em tecido de cancer gastrico preparado em arranjo tecidual em matriz (tma).
• Beneficiário: Celina Tizuko Fujiyama Oshima
• Modalidade de apoio: Auxílio à Pesquisa - Regular