Effects of Cigarette Smoke and Ethanol Intake on Mouse Oesophageal Mucosa Changes Induced by Dietary Zinc Deficiency and Deoxycholic Acid Supplementation

Autor(es)

Zapaterini, Joyce R. ^{[1, 2]} ; de Moura, Nelci A. ^[1] ; Ribeiro, Daniel A. ^[3] ; Rodrigues, Maria A. M. ^[2] ; Barbisan, Luis F. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Inst Biosci, Dept Morphol, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Sch Med, Dept Pathol, Botucatu, SP - Brazil

^[3] Univ Fed Sao Paulo, UNIFESP, Dept Biosci, Santos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY; v. 111, n. 2, p. 92-98, AUG 2012.
• Citações Web of Science: 2

Resumo

The noxious effects of dietary zinc deficiency (ZD) and deoxycholic bile acid (DCA) supplementation in the oesophagus were investigated. The additional influence of cigarette smoke and ethanol intake on the changes in the oesophageal mucosa induced by dietary ZD plus DCA was also assessed. Male C57BL/6 mice were allocated into four groups: Group 1 was fed control diet and groups 24 were fed ZD plus DCA diet. After 5 weeks, groups 3 and 4 were exposed to 10% ethanol intake or cigarette smoke for 15 weeks, respectively. All animals were euthanized at the end of week 20, and the oesophagus, lung, liver and colon were collected and analysed by conventional morphology. Cell proliferation was assessed in the oesophageal mucosa by Ki-67 immunohistochemistry and cyclooxygenase 2 (COX-2) protein by Western blotting. Dietary ZD plus DCA treatment induced mild hyperkeratosis and hyperplasia, increased cell proliferation index and COX-2 protein expression in the oesophagus, and intranuclear inclusion, karyocytomegaly and microvesicular fatty change in the liver. Cigarette smoke increased COX-2 protein expression in oesophageal mucosa and irregular enlargement of alveolus and alveolar ductal air spaces, while ethanol enhanced liver damage induced by ZD plus DCA diet. These findings indicate that dietary ZD plus DCA treatment during 20 weeks induces a pattern of chemical oesophageal injury but not Barrett's-like lesions. (AU)

• Processo FAPESP: 07/57544-1 - Influência da exposição à fumaça do cigarro e ao etanol sobre as alterações da mucosa do esôfago induzidas por dieta modificada em camundongos C57Bl/6
• Beneficiário: Joyce Regina Zapaterini Rossi
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/53366-4 - Fatores ambientais de risco para câncer: análise em modelos experimentais de carcinogênese do trato gastrointestinal. Projeto 1: Exposição à dieta ocidental durante a gestação, lactação, fase juvenil
• Beneficiário: Maria Aparecida Marchesan Rodrigues Kobayasi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/50854-0 - Efeitos do etanol e da fumaça do cigarro no cólon e pulmão de camundongos C57Bl/6 alimentados com dieta deficiente em zinco e suplementada com ácido deoxicólico
• Beneficiário: Nelci Antunes de Moura
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica