Oxidative stress markers and apoptosis in the prostate of diabetic rats and the influence of vitamin C treatment

Autor(es)

Gobbo, Marina Guimaraes ^{[1, 2]} ; Ribeiro, Daniele Lisboa ^[3] ; Taboga, Sebastiao Roberto ^[2] ; de Almeida, Eduardo Alves ^[4] ; Goes, Rejane Maira ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Inst Biol, Dept Cell Biol, Campinas, SP - Brazil

^[2] Univ Estadual Paulisto UNESP, Inst Biosci Humanities & Exact Sci, Dept Biol, Sao Paulo - Brazil

^[3] Fed Univ Uberlandia UFU, Inst Biomed Sci, Histol Sect, Uberlandia, MG - Brazil

^[4] Univ Estadual Paulista UNESP, Inst Biosci Languages & Exact Sci, Dept Chem & Environm Sci, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Cellular Biochemistry; v. 113, n. 7, p. 2223-2233, JUL 2012.
• Citações Web of Science: 11

Resumo

Negative consequences of diabetes on the prostate such as involution are associated with diminished testosterone, insulin deficiency, and hyperglycemia. The contributions of oxidative damage, which usually increases with diabetes, are unknown for these alterations. This study evaluated the impact of streptozotocin-induced diabetes on the biomarkers of the antioxidant system of rat ventral prostate, the influence of vitamin C supplementation on these biomarkers, and on the balance between cell proliferation and death. Diabetes (D) was induced in Wistar male rats by streptozotocin (5?mg/100?g b.w., i.p.). Control animals (C) were injected with a vehicle. Vitamin C (150?mg/kg b.w./day) supplementation was introduced by gavage in diabetes (D?+?V) as well as control (C?+?V) groups. Thirty days after diabetes onset, the rats were killed and the ventral prostates were analyzed using light microscopy, immunocytochemistry, and biochemical assays for biomarkers of oxidative stress. In comparison to control groups, the levels of circulating testosterone, proliferating, and androgen receptor-positive cells decreased in diabetic groups regardless of vitamin C treatment whereas apoptosis was increased. The levels of superoxide dismutase and glutathione peroxidase did not change, but the levels of glutathione-S-transferase (GST) were increased in diabetic prostate. Vitamin C supplementation normalized GST activity and recovered the apoptotic rates in the prostate. In conclusion, GST is a good indicator of compensatory oxidant defense in the prostate at earlier stages of diabetes and vitamin C improves its activity and attenuates apoptosis in the gland. J. Cell. Biochem. 113: 22232233, 2012. (c) 2012 Wiley Periodicals, Inc. (AU)

• Processo FAPESP: 06/03873-1 - Estudo de variações bioquímicas em tilápias, como biomarcadores de contaminação ambiental
• Beneficiário: Eduardo Alves de Almeida
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 08/05341-2 - Repercussões da obesidade na próstata ventral de ratos: caracterização histológica e análise do papel estrogênico
• Beneficiário: Rejane Maira Góes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/00542-0 - Repercussões da obesidade na próstata ventral de ratos: caracterização histológica e análise do papel estrogênico
• Beneficiário: Daniele Lisboa Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/06885-9 - Influência da hiperglicemia e do estresse oxidativo na cinética de proliferação e morte celular no epitélio acinar da próstata de ratos diabéticos
• Beneficiário: Marina Guimarães Gobbo
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/05078-2 - Repercussões da obesidade na próstata ventral de ratos: caracterização histológica e análise do papel estrogênico
• Beneficiário: Samantha Yuri Maeda
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico