The Microbiome of Brazilian Mangrove Sediments as Revealed by Metagenomics

Autor(es):Mostrar menos -

Andreote, Fernando Dini ^[1] ; Javier Jimenez, Diego ^{[2, 3, 4]} ; Chaves, Diego ^[2] ; Franco Dias, Armando Cavalcante ^[1] ; Luvizotto, Danice Mazzer ^[1] ; Dini-Andreote, Francisco ^[1] ; Fasanella, Cristiane Cipola ^[1] ; Lopez, Maryeimy Varon ^[1] ; Baena, Sandra ^{[2, 3]} ; Taketani, Rodrigo Gouvea ^[5] ; de Melo, Itamar Soares ^[5]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Soil Sci, Luiz de Queiroz Coll Agr, Sao Paulo - Brazil

^[2] Colombian Ctr Genom & Bioinformat Extreme Environ, Bogota, Distrito Capita - Colombia

^[3] Pontificia Univ Javeriana, Dept Biol, Bogota, Distrito Capita - Colombia

^[4] Colombian Corp Agr Res CORPO, Ctr Biotechnol & Bioind, Bogota, Distrito Capita - Colombia

^[5] EMBRAPA Environm, Lab Environm Microbiol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 7, n. 6 JUN 21 2012.
• Citações Web of Science: 92

Resumo

Here we embark in a deep metagenomic survey that revealed the taxonomic and potential metabolic pathways aspects of mangrove sediment microbiology. The extraction of DNA from sediment samples and the direct application of pyrosequencing resulted in approximately 215 Mb of data from four distinct mangrove areas (BrMgv01 to 04) in Brazil. The taxonomic approaches applied revealed the dominance of Deltaproteobacteria and Gammaproteobacteria in the samples. Paired statistical analysis showed higher proportions of specific taxonomic groups in each dataset. The metabolic reconstruction indicated the possible occurrence of processes modulated by the prevailing conditions found in mangrove sediments. In terms of carbon cycling, the sequences indicated the prevalence of genes involved in the metabolism of methane, formaldehyde, and carbon dioxide. With respect to the nitrogen cycle, evidence for sequences associated with dissimilatory reduction of nitrate, nitrogen immobilization, and denitrification was detected. Sequences related to the production of adenylsulfate, sulfite, and H2S were relevant to the sulphur cycle. These data indicate that the microbial core involved in methane, nitrogen, and sulphur metabolism consists mainly of Burkholderiaceae, Planctomycetaceae, Rhodobacteraceae, and Desulfobacteraceae. Comparison of our data to datasets from soil and sea samples resulted in the allotment of the mangrove sediments between those samples. The results of this study add valuable data about the composition of microbial communities in mangroves and also shed light on possible transformations promoted by microbial organisms in mangrove sediments. (AU)

• Processo FAPESP: 04/13910-6 - Biodiversidade e atividades funcionais de microrganismos de manguezais do estado de São Paulo
• Beneficiário: Itamar Soares de Melo
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 08/54013-8 - Diversidade de arquéias e bactérias envolvidas na ciclagem do nitrogênio em sedimentos de manguezais
• Beneficiário: Armando Cavalcante Franco Dias
• Modalidade de apoio: Bolsas no Brasil - Doutorado