The Microbiome of Brazilian Mangrove Sediments as Revealed by Metagenomics

Author(s):Show less -

Andreote, Fernando Dini ^[1] ; Javier Jimenez, Diego ^{[2, 3, 4]} ; Chaves, Diego ^[2] ; Franco Dias, Armando Cavalcante ^[1] ; Luvizotto, Danice Mazzer ^[1] ; Dini-Andreote, Francisco ^[1] ; Fasanella, Cristiane Cipola ^[1] ; Lopez, Maryeimy Varon ^[1] ; Baena, Sandra ^{[2, 3]} ; Taketani, Rodrigo Gouvea ^[5] ; de Melo, Itamar Soares ^[5]

Affiliation

^[1] Univ Sao Paulo, Dept Soil Sci, Luiz de Queiroz Coll Agr, Sao Paulo - Brazil

^[2] Colombian Ctr Genom & Bioinformat Extreme Environ, Bogota, Distrito Capita - Colombia

^[3] Pontificia Univ Javeriana, Dept Biol, Bogota, Distrito Capita - Colombia

^[4] Colombian Corp Agr Res CORPO, Ctr Biotechnol & Bioind, Bogota, Distrito Capita - Colombia

^[5] EMBRAPA Environm, Lab Environm Microbiol, Sao Paulo - Brazil

• Document type: Journal article
• Source: PLoS One; v. 7, n. 6 JUN 21 2012.
• Web of Science Citations: 92

Abstract

Here we embark in a deep metagenomic survey that revealed the taxonomic and potential metabolic pathways aspects of mangrove sediment microbiology. The extraction of DNA from sediment samples and the direct application of pyrosequencing resulted in approximately 215 Mb of data from four distinct mangrove areas (BrMgv01 to 04) in Brazil. The taxonomic approaches applied revealed the dominance of Deltaproteobacteria and Gammaproteobacteria in the samples. Paired statistical analysis showed higher proportions of specific taxonomic groups in each dataset. The metabolic reconstruction indicated the possible occurrence of processes modulated by the prevailing conditions found in mangrove sediments. In terms of carbon cycling, the sequences indicated the prevalence of genes involved in the metabolism of methane, formaldehyde, and carbon dioxide. With respect to the nitrogen cycle, evidence for sequences associated with dissimilatory reduction of nitrate, nitrogen immobilization, and denitrification was detected. Sequences related to the production of adenylsulfate, sulfite, and H2S were relevant to the sulphur cycle. These data indicate that the microbial core involved in methane, nitrogen, and sulphur metabolism consists mainly of Burkholderiaceae, Planctomycetaceae, Rhodobacteraceae, and Desulfobacteraceae. Comparison of our data to datasets from soil and sea samples resulted in the allotment of the mangrove sediments between those samples. The results of this study add valuable data about the composition of microbial communities in mangroves and also shed light on possible transformations promoted by microbial organisms in mangrove sediments. (AU)

• FAPESP's process: 04/13910-6 - Biodiversity and functional activities of microorganisms from mangrove of the State of São Paulo
• Grantee: Itamar Soares de Melo
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 08/54013-8 - Diversity of archaea and bacteria involved in the nitrogen cycling in mangrove sediments
• Grantee: Armando Cavalcante Franco Dias
• Support Opportunities: Scholarships in Brazil - Doctorate