Advanced search
Start date
Betweenand
Related content

METATRANSCRIPTOMICS AND GENOMIC CONTEXT IN MICROBIAL COMMUNITIES INVOLVED IN THE BIOGEOCHEMICAL CYCLES IN MANGROVES

Grant number: 12/14534-4
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Start date: November 01, 2012
End date: August 01, 2016
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Fernando Dini Andreote
Grantee:Armando Cavalcante Franco Dias
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated scholarship(s):15/01290-8 - The plasmidome of mangrove soils, BE.EP.PD

Abstract

Among the terrestrial ecosystems, some are special due to the particular combination of environmental conditions, which results in the evolution of species able to colonize these environments. Mangroves compose a biome made of plants, animals and microorganisms that interact in this environment which has as the main characteristic the interface between the continent and the ocean in intertropical regions. Such location creates unique environmental conditions, especially in relation to salinity, frequent anaerobic conditions and seasonal changes in nutrient availability. In mangrove, bacteria, archaea and fungi constitute 91% of the total biomass; however, little is known about the functionality of microbial groups in mangroves. In this context, this project aims to study using innovative approaches (metatranscriptomics and genomic context analysis) the fuctioning of microbial communities in two mangroves located in the State of São Paulo (already targeted in other studies of our group). The gene expression will be determined by extracting RNA directly from sediment samples and further sequencing using large-scale strategies (Illumina and 454), in order to identify genes expressed by the microbial community in mangroves under different states of preservation. Regarding the analysis of genomic context, the aim is to detect genes related to the carbon (mcrA), nitrogen (hzO, nifD, nifH, nirK, nirS, nosZ) and sulfur (aprA, dsrB) cycling in fosmid-libraries already constructed with the DNA from these mangroves, and further sequencing the inserts (average size 20kB), which will allow the connection between the phylogeny and functionality of the microbial groups in the targeted environment. In sum, information will be obtained on these functional communities in an innovative way, not only describing a gene, but also its expression in the mangroves, and the genomic context that it occurs in the microbial communities present in this ecosystem.

News published in Agência FAPESP Newsletter about the scholarship:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA COSTA, DIOGO P.; DIAS, ARMANDO C. F.; COTTA, SIMONE R.; VILELA, DANIELLA; DE ANDRADE, PEDRO A. M.; PELLIZARI, VIVIAN H.; ANDREOTE, FERNANDO DINI. Changes of bacterial communities in the rhizosphere of sugarcane under elevated concentration of atmospheric CO2. Global Change Biology Bioenergy, v. 10, n. 2, p. 137-145, . (11/04857-8, 13/50353-7, 12/14534-4, 11/07343-5)
LIMA-PERIM, JULIA ELIDIA; ROMAGNOLI, EMILIANA MANESCO; DINI-ANDREOTE, FRANCISCO; DURRER, ADEMIR; FRANCO DIAS, ARMANDO CAVALCANTE; ANDREOTE, FERNANDO DINI. Linking the Composition of Bacterial and Archaeal Communities to Characteristics of Soil and Flora Composition in the Atlantic Rainforest. PLoS One, v. 11, n. 1, . (08/50824-1, 12/14534-4)
COTTA, SIMONE RAPOSO; CADETE, LUANA LIRA; VAN ELSAS, JAN DIRK; ANDREOTE, FERNANDO DINI; FRANCO DIAS, ARMANDO CAVALCANTE. Exploring bacterial functionality in mangrove sediments and its capability to overcome anthropogenic activity. Marine Pollution Bulletin, v. 141, p. 586-594, . (12/14534-4, 12/06245-2)
OTTONI, JULIA RONZELLA; CABRAL, LUCELIA; PEREIRA DE SOUSA, SANDERSON TARCISO; LACERDA JUNIOR, GILENO VIEIRA; DOMINGOS, DANIELA FERREIRA; SOARES JUNIOR, FABIO LINO; PINHEIRO DA SILVA, MYLENNE CALCIOLARI; MARCON, JOELMA; FRANCO DIAS, ARMANDO CAVALCANTE; DE MELO, ITAMAR SOARES; et al. Functional metagenomics of oil-impacted mangrove sediments reveals high abundance of hydrolases of biotechnological interest. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 33, n. 7, . (12/16850-0, 12/14534-4, 10/15519-3, 13/09386-9, 13/22555-4)
COTTA, S. R.; CAVALCANTE FRANCO DIAS, A.; SELDIN, L.; ANDREOTE, F. D.; VAN ELSAS, J. D.. The diversity and abundance of phytase genes (beta-propeller phytases) in bacterial communities of the maize rhizosphere. Letters in Applied Microbiology, v. 62, n. 3, p. 264-268, . (12/14534-4)