In vivo hydroquinone exposure impairs MCP-1 secretion and monocyte recruitment into the inflamed lung

Autor(es)

Borges Shimada, Ana Lucia ^[1] ; Teroso Ribeiro, Andre Luiz ^[1] ; Bolonheis, Simone Marques ^[1] ; Ferraz-de-Paula, Viviane ^[1] ; Hebeda, Cristina Bichels ^[1] ; Poliselli Farsky, Sandra Helena ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Lab Expt Toxicol, Dept Clin & Toxicol Anal, Sch Pharmaceut Sci, BR-05508 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicology; v. 296, n. 1-3, p. 20-26, JUN 14 2012.
• Citações Web of Science: 9

Resumo

Alveolar macrophages (AMs) are important cells in the resolution of the inflammatory process and they come into direct contact with inhaled pollutants. Hydroquinone (HQ) is an environmental pollutant and a component of cigarette smoke that causes immunosuppressive effects. In the present work, we showed that mice exposed to low levels of aerosolized HQ (25 ppm; 1 h/day/5 days) presented impaired mononuclear cell migration to the lipopolysaccharide (LPS)-inflamed lung. This may have been due to reduced monocyte chemoattractant protein-1 (MCP-1) secretion into bronchoalveolar lavage fluid (BALF), and it was not related to alterations to mononuclear cell mobilization into the blood or adhesion molecules expression on mononuclear cell membranes. Corroborating the actions of HQ on MCP-1 secretion, reduced MCP-1 concentrations were also found in the supernatant of ex vivo AM and tracheal tissue collected from HQ-exposed mice. A direct action of HQ on MCP-1 secretion, resulting from impaired gene synthesis, was verified by in vitro incubation of naive AMs or tracheal tissue with HQ. The role of reduced levels of MCP-1 in the BALF on monocyte migration was analysed in the human monocytic lineage THP-1 in in vitro chemotaxis assays, which showed that the reduced concentrations of MCP-1 found in the BALF or cell supernatants from HQ-exposed mice impaired cell migration. Considering the fact that MCP-1 presents a broad spectrum of actions on pathophysiological conditions and that resident mononuclear cells are involved in lung tissue homeostasis and in immune host defence, the mechanism of HQ toxicity presented herein might be relevant to the genesis of infectious lung diseases in smokers and in inhabitants of polluted areas. (C) 2012 Elsevier Ireland Ltd. All rights reserved. (AU)

• Processo FAPESP: 09/03964-5 - Caracterização dos Mecanismos de Ação da Exposição à Hidroquinona sobre Mastócitos e Macrófagos Pulmonares.
• Beneficiário: Ana Lucia Borges Shimada
• Modalidade de apoio: Bolsas no Brasil - Mestrado