Differentiation of African Components of Ancestry to Stratify Groups in a Case-Control Study of a Brazilian Urban Population

Autor(es):Mostrar menos -

Silbiger, Vivian N. ^{[1, 2]} ; Hirata, Mario H. ^[1] ; Luchessi, Andre D. ^[1] ; Genvigir, Fabiana D. V. ^[1] ; Cerda, Alvaro ^[1] ; Rodrigues, Alice C. ^[1] ; Willrich, Maria A. V. ^[1] ; Arazi, Simone S. ^[1] ; Dorea, Egidio L. ^[3] ; Bernik, Marcia M. S. ^[3] ; Faludi, Andre A. ^[4] ; Bertolami, Marcelo C. ^[4] ; Santos, Carla ^{[5, 6]} ; Carracedo, Angel ^[5] ; Salas, Antonio ^[5] ; Freire, Ana ^[5] ; Victoria Lareu, Maria ^[5] ; Phillips, Christopher ^[5] ; Porras-Hurtado, Liliana ^[5] ; Fondevila, Manuel ^[5] ; Hirata, Rosario D. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Sao Paulo - Brazil

^[2] Univ Fed Rio Grande do Norte, Dept Clin & Toxicol Anal, BR-59012570 Natal, RN - Brazil

^[3] Univ Sao Paulo, Univ Hosp, Sao Paulo - Brazil

^[4] Inst Dante Pazzanese Cardiol, Sao Paulo - Brazil

^[5] Univ Santiago de Compostela, Inst Legal Med, Forens Genet Unit, Galicia - Spain

^[6] Univ Aveiro, Dept Biol, P-3800 Aveiro - Portugal

• Tipo de documento: Artigo Científico
• Fonte: GENETIC TESTING AND MOLECULAR BIOMARKERS; v. 16, n. 6, p. 524-530, JUN 2012.
• Citações Web of Science: 3

Resumo

Background: Balancing the subject composition of case and control groups to create homogenous ancestries between each group is essential for medical association studies. Methods: We explored the applicability of single-tube 34-plex ancestry informative markers (AIM) single nucleotide polymorphisms (SNPs) to estimate the African Component of Ancestry (ACA) to design a future case-control association study of a Brazilian urban sample. Results: One hundred eighty individuals (107 case group; 73 control group) self-described as white, brown-intermediate or black were selected. The proportions of the relative contribution of a variable number of ancestral population components were similar between case and control groups. Moreover, the case and control groups demonstrated similar distributions for ACA <0.25 and >0.50 categories. Notably a high number of outlier values (23 samples) were observed among individuals with ACA <0.25. These individuals presented a high probability of Native American and East Asian ancestral components; however, no individuals originally giving these self-described ancestries were observed in this study. Conclusions: The strategy proposed for the assessment of ancestry and adjustment of case and control groups for an association study is an important step for the proper construction of the study, particularly when subjects are taken from a complex urban population. This can be achieved using a straight forward multiplexed AIM-SNPs assay of highly discriminatory ancestry markers. (AU)

• Processo FAPESP: 00/12224-0 - Análise das alterações no gene da enzima CYP3A4 do citocromo P450 que influenciam a resposta terapêutica às estatinas em indivíduos hipercolesterolêmicos
• Beneficiário: Rosario Dominguez Crespo Hirata
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/02086-8 - Estudo de polimorfismos genéticos da família ABC: efeitos na expressão gênica e na resposta farmacológica à atorvastatina
• Beneficiário: Rosario Dominguez Crespo Hirata
• Modalidade de apoio: Auxílio à Pesquisa - Regular