Correlation between Clinical/Radiographic Features and Inflammatory Cytokine Networks Produced by Macrophages Stimulated with Endodontic Content

Autor(es)

Martinho, Frederico C. ^[1] ; Chiesa, Wanderson M. M. ^[1] ; Leite, Fabio R. M. ^[2] ; Cirelli, Joni A. ^[3] ; Gomes, Brenda P. F. A. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Restorat Dent, Endodont Div, Piracicaba, SP - Brazil

^[2] Univ Fed Pelotas, Sch Dent, Periodont Div, Dept Semiol & Clin, Pelotas, Rio Grande Do S - Brazil

^[3] State Univ Sao Paulo, Araraquara Dent Sch, Periodont Div, Dept Diag & Oral Surg, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ENDODONTICS; v. 38, n. 6, p. 740-745, JUN 2012.
• Citações Web of Science: 41

Resumo

Introduction: Macrophages are highly activated by endodontic contents. This study investigated the correlation between different clinical signs/symptoms and radiographic features according to the levels of interleukin (IL)-1 beta, tumor necrosis factor alpha (TNF-alpha), IL-6, IL-10, prostaglandin E-2 (PGE(2)), and their networks produced by endodontic content-stimulated macrophages collected from primary endodontic infection with apical periodontitis (PEIAP). Methods: Samples were taken from 21 root canals with PEIAP by using paper points. The presence of exudate (EX), pain on palpation (POP), tenderness to percussion (UP), and the size of the radiographic lesion (SRL) were recorded. Polymerase chain reaction (16S rDNA) was used for bacterial detection and limulus amebocyte lysate (LAL) assay for endotoxin measurement. Raw 264.7 macrophages were stimulated with bacterial contents during 24 hs. The amounts of IL-1 beta, TNF-alpha, IL-6, IL-10 and PGE(2) were measured by enzyme-linked immunosorbent assay. Log-based data were correlated by multiple logistic regression (P < .05). Results: Bacteria and endotoxin were detected in 100% of the samples. IL-6 and TNF-alpha were positively correlated with SRL and EX, respectively (P < .05). Clinical signs/symptoms and radiographic findings were set as dependent variables for EX-positive correlations between PGE(2), IL-1 beta, and TNF-alpha (P < .05), whereas IL-6 and PGE(2) were positively correlated to each other in POP but negatively correlated in SRL (P < .05). When POP and TTP-POP were set as dependent variables, different cytokine networks were found. Conclusions: Our findings suggest different roles for each cytokine in the development of apical periodontitis, whose effects of overlapping networks depend on the signs/symptoms and radiographic features found in endodontic infection. (J Endod 2012;38:740-745) (AU)

• Processo FAPESP: 10/17877-4 - Análise microbiológica, de endoxinas e de citocinas pró-inflamatórias em infecções endodônticas primárias com presença de lesões periapicais
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51113-1 - Efetividade in vitro de soluções irrigadoras e medicações intracanais sobre microrganismos e endotoxina de canais radiculares
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/19136-1 - Estudo microbiológico e de endotoxinas em canais radiculares com necrose pulpar e lesão periapical e avaliação do seu potencial antigênico contra macrófagos na produção de IL1-beta e TNF-alfa durante a terapia endodôntica
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/57551-0 - Microbiota dos canais radiculares de dentes com insucesso do tratamento endodôntico e análise da suscetibilidade antimicrobiana e dos fatores de virulência dos Enterococcus faecalis isolados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/50510-0 - Avaliação in vivo do efeito do preparo químico-mecânico sobre microrganismos e endotoxinas em infecções endodônticas persistentes e suscetibilidade antimicrobiana dos Enterococcus spp. isolados
• Beneficiário: Brenda Paula Figueiredo de Almeida Gomes
• Modalidade de apoio: Auxílio à Pesquisa - Regular