Post-thaw addition of seminal plasma reduces tyrosine phosphorylation on the surface of cryopreserved equine sperm, but does not reduce lipid peroxidation

Autor(es)

Cesar de Andrade, Andre Furugen ^[1] ; Zaffalon, Fabiane Gilli ^[1] ; Carvalho Celeghini, Eneiva Carla ^[1] ; Nascimento, Juliana ^[1] ; Bressan, Fabiana Fernandes ^[2] ; Massami Kitamura Martins, Simone Maria ^{[1, 3]} ; de Arruda, Rubens Paes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo USP, Dept Anim Reprod, Lab Semen Biotechnol & Androl, Sch Vet Med & Anim Sci, Pirassununga, SP - Brazil

^[2] Univ Sao Paulo USP, Lab Mol Morphophysiol & Dev, Dept Basic Sci, Sch Anim Sci & Engn, Pirassununga, SP - Brazil

^[3] Univ Sao Paulo USP, Lab Swine Res, Dept Anim Nutr & Prod, Sch Vet Med & Anim Sci, Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 77, n. 9, p. 1866-1872, JUN 2012.
• Citações Web of Science: 23

Resumo

The objective was to verify the relationship between equine semen cryopreservation and changes related to increased lipid peroxidation. Also, addition of autologous or homologous seminal plasma from a stallion with a good freezing response to post-thawed sperm was tested to determine whether it would confer protection. Frozen-thawed sperm were evaluated and allocated into three groups: without plasma addition, and supplemented with either homologous or autologous seminal plasma. All groups were evaluated at 0, 60 and 120 min after incubation at 37 degrees C. Cryopreservation did not increase plasma membrane disorders (mean +/- SEM 9.48 +/- 0.65 and 1.62 +/- 0.23% in raw and frozen-thawed sperm, respectively). However, both membrane peroxidation and protein phosphorylation were increased (P < 0.05) compared to raw semen (1.74 and 5.20-fold, respectively). There was a correlation (r = 0.73; P < 0.05) between the increase in lipid peroxidation and tyrosine phosphorylation. Seminal plasma, regardless of origin, reduced (P > 0.05) tyrosine phosphorylation present on the surface of cryopreserved sperm; however, lipid peroxidation was not significantly reduced. In conclusion, we inferred that emergence of phosphorylated proteins on the surface of cryopreserved sperm was due to increased lipid peroxidation that occurred during the freezing/thawing process; however, reduced tyrosine phosphorylation that occurred after addition of seminal plasma was triggered by other mechanisms, apparently independent from the reduction in membrane peroxidation. (C) 2012 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 04/09028-6 - Estruturação de parque de equipamentos de ciências celulares e moleculares no campo de Pirassununga
• Beneficiário: Flávio Vieira Meirelles
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 06/60966-2 - Adição do plasma seminal na redução da criocapacitação e da peroxidação lipídica em espermatozóides de equinos
• Beneficiário: André Furugen Cesar de Andrade
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 04/11463-2 - Adicao do plasma seminal na reversao da criocapacitacao e reducao da geracao de especies reativas de oxigenio (ros) em espermatozoides equinos.
• Beneficiário: André Furugen Cesar de Andrade
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 05/60591-6 - Adicao do plasma seminal na reducao da criocapacitacao e da peroxidacao lipidica em espermatozoides de equinos.
• Beneficiário: Rubens Paes de Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Regular