Baccharis dracunculifolia, the main source of green propolis, exhibits potent antioxidant activity and prevents oxidative mitochondrial damage

Autor(es)

Guimaraes, Natalia S. S. ^[1] ; Mello, Joyce C. ^{[1, 2]} ; Paiva, Juliana S. ^[1] ; Bueno, Paula C. P. ^[3] ; Berretta, Andresa A. ^[4] ; Torquato, Ricardo J. ^[5] ; Nantes, Iseli L. ^[2] ; Rodrigues, Tiago ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Mogi das Cruzes UMC, Ctr Interdisciplinar Invest Bioquim CIIB, Mogi Das Cruzes, SP - Brazil

^[2] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas CCNH, Santo Andre, SP - Brazil

^[3] Univ Estadual Paulista UNESP, Inst Quim, Sao Paulo - Brazil

^[4] Fac Ciencias Farmaceut Ribeirao Preto FCFRP USP, Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo UNIFESP, Dept Bioquim, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food and Chemical Toxicology; v. 50, n. 3-4, p. 1091-1097, MAR-APR 2012.
• Citações Web of Science: 40

Resumo

Baccharis dracunculifolia DC (Asteraceae) is the main botanical source used by honeybees to produce Brazilian green propolis whose hepatoprotective properties have been already described. In this work we investigated the protective effects of the glycolic extract of B. dracunculifolia (GEBd) against oxidative stress in isolated rat liver mitochondria (RLM). The GEBd was prepared by fractionated percolation using propylene glycol as solvent. The total phenols and flavonoids, which are substances with recognized antioxidant action, were quantified in GEBd and the phytochemical analysis was carried out by HPLC. GEBd exhibited significant scavenger activity towards DPPH radicals and superoxide anions in a concentration-dependent manner, and also a Fe2+ chelating activity. GEBd decreased the basal H2O2 generation and the Fe2+- or t-BuOOH-induced ROS production in isolated mitochondria. Lipid oxidation of mitochondrial membranes, protein thiol groups and GSH oxidation were also prevented by GEBd. This shows that B. dracunculifolia exhibit potent antioxidant activity protecting liver mitochondria against oxidative damage and such action probably contribute to the antioxidant and hepatoprotective effects of green propolis. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 08/07246-7 - Estudo dos Mecanismos Antioxidantes de Extratos Glicólicos de Baccharis dracunculifolia e Rosmarinus officinalis
• Beneficiário: Natalia Souza Sandim Guimarães
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 08/01724-4 - Caracterização das Propriedades Antioxidantes de Extratos Vegetais contra o Estresse Oxidativo em Mitocôndrias Isoladas e Células em Cultura
• Beneficiário: Tiago Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Regular