Silicon(IV) phthalocyanine-loaded-nanoparticles for application in photodynamic process

Autor(es)

Simioni, Andreza Ribeiro ^[1] ; Primo, Fernando L. ^[2] ; Tedesco, Antonio Claudio ^[2]

Afiliação do(s) autor(es)

^[1] Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, BR-12244000 Sao Jose Dos Campos - Brazil

^[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Grp Fotobiol & Fotomed, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF LASER APPLICATIONS; v. 24, n. 1 FEB 2012.
• Citações Web of Science: 9

Resumo

The aim of this study was to evaluate the potential application of biodegradable nanoparticles containing a photosensitizer in photodynamic therapy. The poly (D,L lactic-co-glycolic acid) nanoparticles were studied by steady-state techniques, time-resolved fluorescence, and laser flash photolysis. The external morphology of the nanoparticles was established by scanning electron microscopy, and the biological activity was evaluated by in vitro cell culture by 3-(4,5 dimethylthiazol-2,5 biphenyl) tetrazolium bromide assay. The particles were spherical in shape exhibiting a 435 nm diameter with a low tendency to aggregate. The loading efficiency was 77%. The phthalocyanine-loaded-nanoparticles maintained their photophysical behavior after encapsulation. The cellular viability was determined, obtaining 70% of cellular death. All the performed physical-chemical, photophysical, and photobiological measurements indicated that the phthalocyanine-loaded-nanoparticles are a promising drug delivery system for photodynamic therapy and photoprocesses. (C) 2012 Laser Institute of America. (AU)

• Processo FAPESP: 08/53719-4 - Utilização de nanocarreadores contendo fármacos fotossensíveis e outros ativos derivados de fatores de crescimento aplicados a processos fotodinâmicos nas áreas da saúde
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/51729-5 - Estudos de fármacos fotossensíveis em modelos tridimensionais de pele em matriz de colágeno: avaliação da ação fotodinâmica no processo de cicatrização tecidual
• Beneficiário: Andreza Ribeiro Simioni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado