| Full text | |
| Author(s): |
Total Authors: 3
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| Affiliation: | [1] Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, BR-12244000 Sao Jose Dos Campos - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Grp Fotobiol & Fotomed, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil
Total Affiliations: 2
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| Document type: | Journal article |
| Source: | JOURNAL OF LASER APPLICATIONS; v. 24, n. 1 FEB 2012. |
| Web of Science Citations: | 9 |
| Abstract | |
The aim of this study was to evaluate the potential application of biodegradable nanoparticles containing a photosensitizer in photodynamic therapy. The poly (D,L lactic-co-glycolic acid) nanoparticles were studied by steady-state techniques, time-resolved fluorescence, and laser flash photolysis. The external morphology of the nanoparticles was established by scanning electron microscopy, and the biological activity was evaluated by in vitro cell culture by 3-(4,5 dimethylthiazol-2,5 biphenyl) tetrazolium bromide assay. The particles were spherical in shape exhibiting a 435 nm diameter with a low tendency to aggregate. The loading efficiency was 77%. The phthalocyanine-loaded-nanoparticles maintained their photophysical behavior after encapsulation. The cellular viability was determined, obtaining 70% of cellular death. All the performed physical-chemical, photophysical, and photobiological measurements indicated that the phthalocyanine-loaded-nanoparticles are a promising drug delivery system for photodynamic therapy and photoprocesses. (C) 2012 Laser Institute of America. (AU) | |
| FAPESP's process: | 08/53719-4 - Use of nanocarriers containing photosensitive dyes and other derived and growing factors applied to the photodynamic process in the health areas |
| Grantee: | Antonio Claudio Tedesco |
| Support Opportunities: | Research Projects - Thematic Grants |