Detection of Helicobacter pylori in gastric biopsies, saliva and dental plaques of dyspeptic patients from Marília, São Paulo, Brazil: presence of vacA and cagA genes

Autor(es)

Rasmussen, L. T. ^[1] ; de Labio, R. W. ^[2] ; Neto, A. C. ^[2] ; Silva, L. C. ^[3] ; Queiroz, V. F. ^[4] ; Smith, M. A. C. ^[5] ; Payao, S. L. M. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sagrado Coracao, Postgrad Program Oral Biol, Bauru, SP - Brazil

^[2] Marilia Med Sch FAMEMA, Dept Genet, FAMEMA Blood Ctr, Marilia, SP - Brazil

^[3] Marilia Med Sch FAMEMA, Dept Anat Pathol, Marilia, SP - Brazil

^[4] Marilia Med Sch FAMEMA, Dept Digest Syst Surg, Marilia, SP - Brazil

^[5] Univ Fed Sao Paulo UNIFESP, Dept Morphol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Venomous Animals and Toxins including Tropical Diseases; v. 18, n. 2, p. 180-187, 2012.
• Citações Web of Science: 6

Resumo

Helicobacter pylori, a gram-negative bacterium, possesses two important virulence factors: the vacuolating toxin (vacA), and the cytotoxin-associated gene product (cagA). The aim of the present study was to evaluate the presence of H. pylori in the stomach and oral cavity of humans and compare the cagA and vacA genotypes of H. pylori found in different samples (stomach, saliva and dental plaque) from the same patient. Gastric biopsies, saliva and dental plaques were obtained from 62 dyspeptic adults. DNA was extracted and evaluated for the presence of H. pylori and the alleles cagA and vacA. Persons with gastritis had a higher frequency of H. pylori -positive samples in the stomach while positive samples from gastric biopsies were significantly correlated with those from the oral cavity. There was a high H. pylori frequency in patients while the cagA gene was associated with vacA s1 alleles in gastric biopsies. Our results suggest a reservoir of the species in the oral cavity and that, in one patient, more than one H. pylori strain may exist in the saliva, dental plaque and stomach. We found a relationship between gastric infection and the bacterium in the oral cavity, with the cytotoxin genotype varying between saliva and dental plaque. (AU)

• Processo FAPESP: 06/60836-1 - Caracterizacao dos marcadores de patogenicidade caga e vaca em amostras de placa dental, saliva e mucosa gastrica de pacientes com gastrite.
• Beneficiário: Spencer Luiz Marques Payão
• Modalidade de apoio: Auxílio à Pesquisa - Regular