Functional and small-angle X-ray scattering studies of a new stationary phase survival protein E (SurE) from Xylella fastidiosa - evidence of allosteric behaviour

Autor(es)

Saraiva, Antonio M. ^[1] ; Reis, Marcelo A. ^{[2, 3]} ; Tada, Susely F. ^[1] ; Rosselli-Murai, Luciana K. ^[1] ; Schneider, Dilaine R. S. ^[1] ; Pelloso, Alexandre C. ^[1] ; Toledo, Marcelo A. S. ^[1] ; Giles, Carlos ^[2] ; Aparicio, Ricardo ^[3] ; de Souza, Anete P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, CBMEG, Sao Paulo - Brazil

^[2] Univ Estadual Campinas, Inst Fis Gleb Wataghin, Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Inst Quim, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEBS Journal; v. 276, n. 22, p. 6751-6762, 2009.
• Citações Web of Science: 6

Resumo

The genome data of bacterium Xylella fastidiosa strain 9a5c has identified several orfs related to its phytopathogenic adaptation and survival. Among these genes, the surE codifies a survival protein E (XfSurE) whose function is not so well understood, but functional assays in Escherichia coli revealed nucleotidase and exopolyphosphate activity. In the present study, we report the XfSurE protein overexpression in E. coli and its purification. The overall secondary structure was analyzed by CD. Small-angle X-ray scattering and gel. filtration techniques demonstrated that the oligomeric state of the protein in solution is a tetramer. In addition, functional kinetics experiments were carried out with several monophosphate nucleoside substrates and revealed a highly positive cooperativity. An allosteric mechanism involving torsion movements in solution is proposed to explain the cooperative behaviour of XfSurE. This is the. first characterization of a SurE enzyme from a phytopathogen organism and, to our knowledge, the. first solution structure of a SurE protein to be described. (AU)

• Processo FAPESP: 01/07533-7 - Resolução da estrutura tridimensional de proteínas relacionadas à patogenicidade de Xyllela fastidiosa
• Beneficiário: Anete Pereira de Souza
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 05/03234-6 - Estudos estruturais da LGI1 humana
• Beneficiário: Ricardo Aparicio
• Modalidade de apoio: Auxílio à Pesquisa - Regular